Live imaging and quantitation of insect feeding-induced Ca(2+) signal using GCaMP3-based system in Nicotiana benthamiana

Wounding evokes transient increases in cytosolic calcium (Ca(2+)) concentration. Visualizing real-time Ca(2+) flux provides new insights into Ca(2+)-signaling pathways. Here, we outline a protocol to detect insect feeding-induced Ca(2+) flux elevation in Nicotiana benthamiana leaves based on the GCa...

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Detalles Bibliográficos
Autores principales: Wang, Yunjing, Gong, Qian, Huang, Fan, He, Linfang, Liu, Yule
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8689350/
https://www.ncbi.nlm.nih.gov/pubmed/34977683
http://dx.doi.org/10.1016/j.xpro.2021.101040

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8689350/
https://www.ncbi.nlm.nih.gov/pubmed/34977683
http://dx.doi.org/10.1016/j.xpro.2021.101040

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