Cargando…
Live imaging and quantitation of insect feeding-induced Ca(2+) signal using GCaMP3-based system in Nicotiana benthamiana
Wounding evokes transient increases in cytosolic calcium (Ca(2+)) concentration. Visualizing real-time Ca(2+) flux provides new insights into Ca(2+)-signaling pathways. Here, we outline a protocol to detect insect feeding-induced Ca(2+) flux elevation in Nicotiana benthamiana leaves based on the GCa...
| Autores principales: | , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8689350/ https://www.ncbi.nlm.nih.gov/pubmed/34977683 http://dx.doi.org/10.1016/j.xpro.2021.101040 |