A Protein-Engineered, Enhanced Yeast Display Platform for Rapid Evolution of Challenging Targets

[Image: see text] Here, we enhanced the popular yeast display method by multiple rounds of DNA and protein engineering. We introduced surface exposure-tailored reporters, eUnaG2 and DnbALFA, creating a new platform of C and N terminal fusion vectors. The optimization of eUnaG2 resulted in five times...

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Detalles Bibliográficos
Autores principales: Zahradník, Jiří, Dey, Debabrata, Marciano, Shir, Kolářová, Lucie, Charendoff, Chloé I., Subtil, Agathe, Schreiber, Gideon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8689690/
https://www.ncbi.nlm.nih.gov/pubmed/34809429
http://dx.doi.org/10.1021/acssynbio.1c00395

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8689690/
https://www.ncbi.nlm.nih.gov/pubmed/34809429
http://dx.doi.org/10.1021/acssynbio.1c00395

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