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Super-enhancer switching drives a burst in gene expression at the mitosis-to-meiosis transition
Due to bursts in the expression of thousands of germline-specific genes, the testis has the most diverse and complex transcriptome of all organs. By analyzing the male germline of mice, we demonstrate that the genome-wide reorganization of super-enhancers (SEs) drives bursts in germline gene express...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8690596/ https://www.ncbi.nlm.nih.gov/pubmed/32895557 http://dx.doi.org/10.1038/s41594-020-0488-3 |
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author | Maezawa, So Sakashita, Akihiko Yukawa, Masashi Chen, Xiaoting Takahashi, Kazuki Alavattam, Kris G. Nakata, Ippo Weirauch, Matthew T. Barski, Artem Namekawa, Satoshi H. |
author_facet | Maezawa, So Sakashita, Akihiko Yukawa, Masashi Chen, Xiaoting Takahashi, Kazuki Alavattam, Kris G. Nakata, Ippo Weirauch, Matthew T. Barski, Artem Namekawa, Satoshi H. |
author_sort | Maezawa, So |
collection | PubMed |
description | Due to bursts in the expression of thousands of germline-specific genes, the testis has the most diverse and complex transcriptome of all organs. By analyzing the male germline of mice, we demonstrate that the genome-wide reorganization of super-enhancers (SEs) drives bursts in germline gene expression after the mitosis-to-meiosis transition. SE reorganization is regulated by two molecular events: the establishment of meiosis-specific SEs via A-MYB (MYBL1), a key transcription factor for germline genes, and the resolution of SEs in mitotically proliferating cells via SCML2, a germline-specific Polycomb protein required for spermatogenesis-specific gene expression. Prior to entry into meiosis, meiotic SEs are preprogrammed in mitotic spermatogonia to ensure the unidirectional differentiation of spermatogenesis. We identify key regulatory factors for both mitotic and meiotic enhancers, revealing a molecular logic for the concurrent activation of mitotic enhancers and suppression of meiotic enhancers in the somatic and/or mitotic proliferation phases. |
format | Online Article Text |
id | pubmed-8690596 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
record_format | MEDLINE/PubMed |
spelling | pubmed-86905962021-12-21 Super-enhancer switching drives a burst in gene expression at the mitosis-to-meiosis transition Maezawa, So Sakashita, Akihiko Yukawa, Masashi Chen, Xiaoting Takahashi, Kazuki Alavattam, Kris G. Nakata, Ippo Weirauch, Matthew T. Barski, Artem Namekawa, Satoshi H. Nat Struct Mol Biol Article Due to bursts in the expression of thousands of germline-specific genes, the testis has the most diverse and complex transcriptome of all organs. By analyzing the male germline of mice, we demonstrate that the genome-wide reorganization of super-enhancers (SEs) drives bursts in germline gene expression after the mitosis-to-meiosis transition. SE reorganization is regulated by two molecular events: the establishment of meiosis-specific SEs via A-MYB (MYBL1), a key transcription factor for germline genes, and the resolution of SEs in mitotically proliferating cells via SCML2, a germline-specific Polycomb protein required for spermatogenesis-specific gene expression. Prior to entry into meiosis, meiotic SEs are preprogrammed in mitotic spermatogonia to ensure the unidirectional differentiation of spermatogenesis. We identify key regulatory factors for both mitotic and meiotic enhancers, revealing a molecular logic for the concurrent activation of mitotic enhancers and suppression of meiotic enhancers in the somatic and/or mitotic proliferation phases. 2020-09-07 2020-10 /pmc/articles/PMC8690596/ /pubmed/32895557 http://dx.doi.org/10.1038/s41594-020-0488-3 Text en http://www.nature.com/authors/editorial_policies/license.html#termsUsers may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Maezawa, So Sakashita, Akihiko Yukawa, Masashi Chen, Xiaoting Takahashi, Kazuki Alavattam, Kris G. Nakata, Ippo Weirauch, Matthew T. Barski, Artem Namekawa, Satoshi H. Super-enhancer switching drives a burst in gene expression at the mitosis-to-meiosis transition |
title | Super-enhancer switching drives a burst in gene expression at the mitosis-to-meiosis transition |
title_full | Super-enhancer switching drives a burst in gene expression at the mitosis-to-meiosis transition |
title_fullStr | Super-enhancer switching drives a burst in gene expression at the mitosis-to-meiosis transition |
title_full_unstemmed | Super-enhancer switching drives a burst in gene expression at the mitosis-to-meiosis transition |
title_short | Super-enhancer switching drives a burst in gene expression at the mitosis-to-meiosis transition |
title_sort | super-enhancer switching drives a burst in gene expression at the mitosis-to-meiosis transition |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8690596/ https://www.ncbi.nlm.nih.gov/pubmed/32895557 http://dx.doi.org/10.1038/s41594-020-0488-3 |
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