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Endogenous protein tagging in medaka using a simplified CRISPR/Cas9 knock-in approach
The CRISPR/Cas9 system has been used to generate fluorescently labelled fusion proteins by homology-directed repair in a variety of species. Despite its revolutionary success, there remains an urgent need for increased simplicity and efficiency of genome editing in research organisms. Here, we estab...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
eLife Sciences Publications, Ltd
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8691840/ https://www.ncbi.nlm.nih.gov/pubmed/34870593 http://dx.doi.org/10.7554/eLife.75050 |