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Isolation of Primary Mouse Pulmonary Microvascular Endothelial Cells and Generation of an Immortalized Cell Line to Obtain Sufficient Extracellular Vesicles

Pulmonary microvascular endothelial cells (PMECs) and the extracellular vesicles (EVs) derived from PMECs participate in maintaining pulmonary homeostasis and mediating the inflammatory response. However, obtaining a high-purity population of PMECs and their EVs from mouse is still notoriously diffi...

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Detalles Bibliográficos
Autores principales: Liu, Xu, Xia, Feiping, Wu, Xiao, Tang, Ying, Wang, Lu, Sun, Qin, Xue, Ming, Chang, Wei, Liu, Ling, Guo, Fengmei, Yang, Yi, Qiu, Haibo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8692730/
https://www.ncbi.nlm.nih.gov/pubmed/34956190
http://dx.doi.org/10.3389/fimmu.2021.759176