A Stabilized, Monomeric, Receptor Binding Domain Elicits High-Titer Neutralizing Antibodies Against All SARS-CoV-2 Variants of Concern

Saturation suppressor mutagenesis was used to generate thermostable mutants of the SARS-CoV-2 spike receptor-binding domain (RBD). A triple mutant with an increase in thermal melting temperature of ~7°C with respect to the wild-type B.1 RBD and was expressed in high yield in both mammalian cells and...

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Autores principales: Ahmed, Shahbaz, Khan, Mohammad Suhail, Gayathri, Savitha, Singh, Randhir, Kumar, Sahil, Patel, Unnatiben Rajeshbhai, Malladi, Sameer Kumar, Rajmani, Raju S., van Vuren, Petrus Jansen, Riddell, Shane, Goldie, Sarah, Girish, Nidhi, Reddy, Poorvi, Upadhyaya, Aditya, Pandey, Suman, Siddiqui, Samreen, Tyagi, Akansha, Jha, Sujeet, Pandey, Rajesh, Khatun, Oyahida, Narayan, Rohan, Tripathi, Shashank, McAuley, Alexander J., Singanallur, Nagendrakumar Balasubramanian, Vasan, Seshadri S., Ringe, Rajesh P., Varadarajan, Raghavan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Immunology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8695907/
https://www.ncbi.nlm.nih.gov/pubmed/34956193
http://dx.doi.org/10.3389/fimmu.2021.765211

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8695907/
https://www.ncbi.nlm.nih.gov/pubmed/34956193
http://dx.doi.org/10.3389/fimmu.2021.765211

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