Impact of Freezing on the Microbiological Quality and Physical Characteristics of Buffalo Mozzarella Cheese

SIMPLE SUMMARY: Buffalo Mozzarella cheese from Campania is made from the fresh milk of the Italian Mediterranean buffalo. In 1996, thanks to its distinctive characteristics (specific environmental conditions and production method), Regulation (EC) No. 1107/96 recognized it as a Protected Designation of Origin product. The limited availability of milk and the increased demand for buffalo mozzarella cheese, especially during the spring-summer period (when milk production is lowest), have induced the use of frozen milk or curd for its production. The aim of this research was to provide preliminary results about the effect of freezing on microbial communities of fresh buffalo milk, curd and Buffalo Mozzarella cheese, and on physical properties (whiteness, hardness, and oxidation state) of Buffalo Mozzarella cheese. The preliminary results obtained have allowed us to conclude that the freezing process if properly carried out, does not compromise the microbiological quality of the products but produces only slight changes of some physical properties. ABSTRACT: Buffalo Mozzarella cheese from Campania is one of the most worldwide appreciated Italian dairy products. The increased demand for buffalo dairy products and the limited availability of the finest buffalo milk has prompted the diffusion of illicit practices, such as the use of milk, curd, or other products that are frozen or bought at low cost. The aim of this research was to provide preliminary results about the trend of the microbial communities of buffalo milk, curd and Buffalo Mozzarella cheese, during freezing storage of eleven months. At the same time, the alterations of physical properties and the presence of the molecular marker “γ4-casein”, have been investigated. The results showed that freezing reduced the concentrations of the total bacterial count, Enterobacteriaceae, coliforms, Escherichia coli and yeasts in fresh milk and, the concentrations of the total bacterial count, coliforms, lactic acid bacteria and yeasts in mature curd. In the finished product, no notable decreases were observed, except for lactic acid bacteria. About the γ4-casein, no increase was observed in all matrices. These preliminary results allow us to conclude that the freezing process if properly carried out, does not compromise the microbiological quality and the physical properties of the Buffalo Mozzarella cheese.