Cargando…

Heterologous Expression and Rational Design of l-asparaginase from Rhizomucor miehei to Improve Thermostability

SIMPLE SUMMARY: l-asparaginase has been extensively applied in food industries. However, the application of l-asparaginase from non-thermophilic sources is greatly limited due to the poor thermostability and the complex environments typically encountered in food industries. Therefore, improving the...

Descripción completa

Detalles Bibliográficos
Autores principales:	Zhang, Xian, Wang, Zhi, Wang, Yimai, Li, Xu, Zhu, Manchi, Zhang, Hengwei, Xu, Meijuan, Yang, Taowei, Rao, Zhiming
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8698271/ https://www.ncbi.nlm.nih.gov/pubmed/34943261 http://dx.doi.org/10.3390/biology10121346

Ejemplares similares

Simultaneous cell disruption and semi-quantitative activity assays for high-throughput screening of thermostable L-asparaginases
por: Li, Xu, et al.
Publicado: (2018)

Genome sequence and transcriptome analyses of the thermophilic zygomycete fungus Rhizomucor miehei
por: Zhou, Peng, et al.
Publicado: (2014)

Structure-Guided Modification of Rhizomucor miehei Lipase for Production of Structured Lipids
por: Zhang, Jun-Hui, et al.
Publicado: (2013)

Optimization of the Fermentative Production of Rhizomucor miehei Lipase in Aspergillus oryzae by Controlling Morphology
por: Li, Chao, et al.
Publicado: (2022)

Correction: Structure-Guided Modification of Rhizomucor miehei Lipase for Production of Structured Lipids
por: Zhang, Jun-Hui, et al.
Publicado: (2013)

Structural insights into the substrate specificity of two esterases from the thermophilic Rhizomucor miehei
por: Yang, Shaoqing, et al.
Publicado: (2015)

Enantioselective transacetylation of (R,S)-β-citronellol by propanol rinsed immobilized Rhizomucor miehei lipase
por: Majumder, Abir B, et al.
Publicado: (2007)

Safety evaluation of the food enzyme mucorpepsin from Rhizomucor miehei strain DSM 29547
por: Lambré, Claude, et al.
Publicado: (2022)

Study the influence of culture conditions on rennin production by Rhizomucor miehei using solid-state fermentations
por: Aljammas, Houthail Alahmad, et al.
Publicado: (2018)

Biochemical Characterization of a First Fungal Esterase from Rhizomucor miehei Showing High Efficiency of Ester Synthesis
por: Liu, Yu, et al.
Publicado: (2013)

Novel Inhibitory Function of the Rhizomucor miehei Lipase Propeptide and Three-Dimensional Structures of Its Complexes with the Enzyme
por: Moroz, Olga V., et al.
Publicado: (2019)

Optimization of protease production from Rhizomucor miehei Rm4 isolate under solid-state fermentation
por: Alahmad Aljammas, Houthail, et al.
Publicado: (2022)

Safety evaluation of the thermolabile form of the food enzyme mucorpepsin from Rhizomucor miehei strain MMR 164
por: Lambré, Claude, et al.
Publicado: (2022)

Directed evolution of a β-mannanase from Rhizomucor miehei to improve catalytic activity in acidic and thermophilic conditions
por: Li, Yan-xiao, et al.
Publicado: (2017)

Safety evaluation of the native and thermolabile forms of the food enzyme mucorpepsin from Rhizomucor miehei strain MMR 164
por: Lambré, Claude, et al.
Publicado: (2022)

Improved production of recombinant Rhizomucor miehei lipase by coexpressing protein folding chaperones in Pichia pastoris, which triggered ER stress
por: Huang, Jinjin, et al.
Publicado: (2020)

Sustainable isomaltulose production in Corynebacterium glutamicum by engineering the thermostability of sucrose isomerase coupled with one-step simplified cell immobilization
por: Hu, Mengkai, et al.
Publicado: (2022)

Characterization of a Novel Aspartic Protease from Rhizomucor miehei Expressed in Aspergillus niger and Its Application in Production of ACE-Inhibitory Peptides
por: Wang, Shounan, et al.
Publicado: (2021)

Fine Modulation of the Catalytic Properties of Rhizomucor miehei Lipase Driven by Different Immobilization Strategies for the Selective Hydrolysis of Fish Oil
por: Yousefi, Maryam, et al.
Publicado: (2020)

Kinetic Modeling, Thermodynamic Approach and Molecular Dynamics Simulation of Thermal Inactivation of Lipases from Burkholderia cepacia and Rhizomucor miehei
por: Ortega, Natividad, et al.
Publicado: (2022)

Improved production of a recombinant Rhizomucor miehei lipase expressed in Pichia pastoris and its application for conversion of microalgae oil to biodiesel
por: Huang, Jinjin, et al.
Publicado: (2014)

Improved methanol tolerance of Rhizomucor miehei lipase based on N‑glycosylation within the α-helix region and its application in biodiesel production
por: Tian, Miao, et al.
Publicado: (2021)

Lipase From Rhizomucor miehei Immobilized on Magnetic Nanoparticles: Performance in Fatty Acid Ethyl Ester (FAEE) Optimized Production by the Taguchi Method
por: Moreira, Katerine da S., et al.
Publicado: (2020)

Engineered disulfide bonds improve thermostability and activity of L‐isoleucine hydroxylase for efficient 4‐HIL production in Bacillus subtilis 168
por: Qiao, Zhina, et al.
Publicado: (2019)

A Novel 3-Phytosterone-9α-Hydroxylase Oxygenation Component and Its Application in Bioconversion of 4-Androstene-3,17-Dione to 9α-Hydroxy-4-Androstene-3,17-Dione Coupling with A NADH Regeneration Formate Dehydrogenase
por: Zhang, Xian, et al.
Publicado: (2019)

Synthetic engineering of Corynebacterium crenatum to selectively produce acetoin or 2,3-butanediol by one step bioconversion method
por: Zhang, Xian, et al.
Publicado: (2019)

Modified coptisine derivatives as an inhibitor against pathogenic Rhizomucor miehei, Mycolicibacterium smegmatis (Black Fungus), Monkeypox, and Marburg virus by molecular docking and molecular dynamics simulation-based drug design approach
por: Akash, Shopnil, et al.
Publicado: (2023)

Efficient 9α-hydroxy-4-androstene-3,17-dione production by engineered Bacillus subtilis co-expressing Mycobacterium neoaurum 3-ketosteroid 9α-hydroxylase and B. subtilis glucose 1-dehydrogenase with NADH regeneration
por: Zhang, Xian, et al.
Publicado: (2016)

Elimination of a Free Cysteine by Creation of a Disulfide Bond Increases the Activity and Stability of Candida boidinii Formate Dehydrogenase
por: Zheng, Junxian, et al.
Publicado: (2016)

Biochemical Characterization and Structural Insight into Interaction and Conformation Mechanisms of Serratia marcescens Lysine Decarboxylase (SmcadA)
por: Osire, Tolbert, et al.
Publicado: (2021)

Engineering of microbial cells for L-valine production: challenges and opportunities
por: Gao, Hui, et al.
Publicado: (2021)

Improved Production of 2,3-Butanediol in Bacillus amyloliquefaciens by Over-Expression of Glyceraldehyde-3-Phosphate Dehydrogenase and 2,3-butanediol Dehydrogenase
por: Yang, Taowei, et al.
Publicado: (2013)

Enhanced 2,3-butanediol production from biodiesel-derived glycerol by engineering of cofactor regeneration and manipulating carbon flux in Bacillus amyloliquefaciens
por: Yang, Taowei, et al.
Publicado: (2015)

Enhanced Production of Androst-1,4-Diene-3,17-Dione by Mycobacterium neoaurum JC-12 Using Three-Stage Fermentation Strategy
por: Shao, Minglong, et al.
Publicado: (2015)

Systems pathway engineering of Corynebacterium crenatum for improved L-arginine production
por: Man, Zaiwei, et al.
Publicado: (2016)

Effect of Polyhydroxybutyrate (PHB) storage on l-arginine production in recombinant Corynebacterium crenatum using coenzyme regulation
por: Xu, Meijuan, et al.
Publicado: (2016)

Cloning of a novel thermostable glucoamylase from thermophilic fungus Rhizomucor pusillus and high-level co-expression with α-amylase in Pichia pastoris
por: He, Zhenggui, et al.
Publicado: (2014)

Intracellular Environment Improvement of Mycobacterium neoaurum for Enhancing Androst-1,4-Diene-3,17-Dione Production by Manipulating NADH and Reactive Oxygen Species Levels
por: Shao, Minglong, et al.
Publicado: (2019)

Improving prodigiosin production by transcription factor engineering and promoter engineering in Serratia marcescens
por: Pan, Xuewei, et al.
Publicado: (2022)

N20D/N116E Combined Mutant Downward Shifted the pH Optimum of Bacillus subtilis NADH Oxidase
por: Yang, Taowei, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_lt878b8plp1095vs0u51elkdt5