Cargando…

Lactobacillus rhamnosus attenuates Thai chili extracts induced gut inflammation and dysbiosis despite capsaicin bactericidal effect against the probiotics, a possible toxicity of high dose capsaicin

Because of a possible impact of capsaicin in the high concentrations on enterocyte injury (cytotoxicity) and bactericidal activity on probiotics, Lactobacillus rhamnosus L34 (L34) and Lactobacillus rhamnosus GG (LGG), the probiotics derived from Thai and Caucasian population, respectively, were test...

Descripción completa

Detalles Bibliográficos
Autores principales: Panpetch, Wimonrat, Visitchanakun, Peerapat, Saisorn, Wilasinee, Sawatpanich, Ajcharaporn, Chatthanathon, Piraya, Somboonna, Naraporn, Tumwasorn, Somying, Leelahavanichkul, Asada
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8699716/
https://www.ncbi.nlm.nih.gov/pubmed/34941893
http://dx.doi.org/10.1371/journal.pone.0261189
_version_ 1784620580605001728
author Panpetch, Wimonrat
Visitchanakun, Peerapat
Saisorn, Wilasinee
Sawatpanich, Ajcharaporn
Chatthanathon, Piraya
Somboonna, Naraporn
Tumwasorn, Somying
Leelahavanichkul, Asada
author_facet Panpetch, Wimonrat
Visitchanakun, Peerapat
Saisorn, Wilasinee
Sawatpanich, Ajcharaporn
Chatthanathon, Piraya
Somboonna, Naraporn
Tumwasorn, Somying
Leelahavanichkul, Asada
author_sort Panpetch, Wimonrat
collection PubMed
description Because of a possible impact of capsaicin in the high concentrations on enterocyte injury (cytotoxicity) and bactericidal activity on probiotics, Lactobacillus rhamnosus L34 (L34) and Lactobacillus rhamnosus GG (LGG), the probiotics derived from Thai and Caucasian population, respectively, were tested in the chili-extract administered C57BL/6 mice and in vitro experiments. In comparison with placebo, 2 weeks administration of the extract from Thai chili in mice caused loose feces and induced intestinal permeability defect as indicated by FITC-dextran assay and the reduction in tight junction molecules (occludin and zona occludens-1) using fluorescent staining and gene expression by quantitative real-time polymerase chain reaction (qRT-PCR). Additionally, the chili extracts also induced the translocation of gut pathogen molecules; lipopolysaccharide (LPS) and (1→3)-β-d-glucan (BG) and fecal dysbiosis (microbiome analysis), including reduced Firmicutes, increased Bacteroides, and enhanced total Gram-negative bacteria in feces. Both L34 and LGG attenuated gut barrier defect (FITC-dextran, the fluorescent staining and gene expression of tight junction molecules) but not improved fecal consistency. Additionally, high concentrations of capsaicin (0.02–2 mM) damage enterocytes (Caco-2 and HT-29) as indicated by cell viability test, supernatant cytokine (IL-8), transepithelial electrical resistance (TEER) and transepithelial FITC-dextran (4.4 kDa) but were attenuated by Lactobacillus condition media (LCM) from both probiotic-strains. The 24 h incubation with 2 mM capsaicin (but not the lower concentrations) reduced the abundance of LGG (but not L34) implying a higher capsaicin tolerance of L34. However, Lactobacillus rhamnosus fecal abundance, using qRT-PCR, of L34 or LGG after 3, 7, and 20 days of the administration in the Thai healthy volunteers demonstrated the similarity between both strains. In conclusion, high dose chili extracts impaired gut permeability and induced gut dysbiosis but were attenuated by probiotics. Despite a better capsaicin tolerance of L34 compared with LGG in vitro, L34 abundance in feces was not different to LGG in the healthy volunteers. More studies on probiotics with a higher intake of chili in human are interesting.
format Online
Article
Text
id pubmed-8699716
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-86997162021-12-24 Lactobacillus rhamnosus attenuates Thai chili extracts induced gut inflammation and dysbiosis despite capsaicin bactericidal effect against the probiotics, a possible toxicity of high dose capsaicin Panpetch, Wimonrat Visitchanakun, Peerapat Saisorn, Wilasinee Sawatpanich, Ajcharaporn Chatthanathon, Piraya Somboonna, Naraporn Tumwasorn, Somying Leelahavanichkul, Asada PLoS One Research Article Because of a possible impact of capsaicin in the high concentrations on enterocyte injury (cytotoxicity) and bactericidal activity on probiotics, Lactobacillus rhamnosus L34 (L34) and Lactobacillus rhamnosus GG (LGG), the probiotics derived from Thai and Caucasian population, respectively, were tested in the chili-extract administered C57BL/6 mice and in vitro experiments. In comparison with placebo, 2 weeks administration of the extract from Thai chili in mice caused loose feces and induced intestinal permeability defect as indicated by FITC-dextran assay and the reduction in tight junction molecules (occludin and zona occludens-1) using fluorescent staining and gene expression by quantitative real-time polymerase chain reaction (qRT-PCR). Additionally, the chili extracts also induced the translocation of gut pathogen molecules; lipopolysaccharide (LPS) and (1→3)-β-d-glucan (BG) and fecal dysbiosis (microbiome analysis), including reduced Firmicutes, increased Bacteroides, and enhanced total Gram-negative bacteria in feces. Both L34 and LGG attenuated gut barrier defect (FITC-dextran, the fluorescent staining and gene expression of tight junction molecules) but not improved fecal consistency. Additionally, high concentrations of capsaicin (0.02–2 mM) damage enterocytes (Caco-2 and HT-29) as indicated by cell viability test, supernatant cytokine (IL-8), transepithelial electrical resistance (TEER) and transepithelial FITC-dextran (4.4 kDa) but were attenuated by Lactobacillus condition media (LCM) from both probiotic-strains. The 24 h incubation with 2 mM capsaicin (but not the lower concentrations) reduced the abundance of LGG (but not L34) implying a higher capsaicin tolerance of L34. However, Lactobacillus rhamnosus fecal abundance, using qRT-PCR, of L34 or LGG after 3, 7, and 20 days of the administration in the Thai healthy volunteers demonstrated the similarity between both strains. In conclusion, high dose chili extracts impaired gut permeability and induced gut dysbiosis but were attenuated by probiotics. Despite a better capsaicin tolerance of L34 compared with LGG in vitro, L34 abundance in feces was not different to LGG in the healthy volunteers. More studies on probiotics with a higher intake of chili in human are interesting. Public Library of Science 2021-12-23 /pmc/articles/PMC8699716/ /pubmed/34941893 http://dx.doi.org/10.1371/journal.pone.0261189 Text en © 2021 Panpetch et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Panpetch, Wimonrat
Visitchanakun, Peerapat
Saisorn, Wilasinee
Sawatpanich, Ajcharaporn
Chatthanathon, Piraya
Somboonna, Naraporn
Tumwasorn, Somying
Leelahavanichkul, Asada
Lactobacillus rhamnosus attenuates Thai chili extracts induced gut inflammation and dysbiosis despite capsaicin bactericidal effect against the probiotics, a possible toxicity of high dose capsaicin
title Lactobacillus rhamnosus attenuates Thai chili extracts induced gut inflammation and dysbiosis despite capsaicin bactericidal effect against the probiotics, a possible toxicity of high dose capsaicin
title_full Lactobacillus rhamnosus attenuates Thai chili extracts induced gut inflammation and dysbiosis despite capsaicin bactericidal effect against the probiotics, a possible toxicity of high dose capsaicin
title_fullStr Lactobacillus rhamnosus attenuates Thai chili extracts induced gut inflammation and dysbiosis despite capsaicin bactericidal effect against the probiotics, a possible toxicity of high dose capsaicin
title_full_unstemmed Lactobacillus rhamnosus attenuates Thai chili extracts induced gut inflammation and dysbiosis despite capsaicin bactericidal effect against the probiotics, a possible toxicity of high dose capsaicin
title_short Lactobacillus rhamnosus attenuates Thai chili extracts induced gut inflammation and dysbiosis despite capsaicin bactericidal effect against the probiotics, a possible toxicity of high dose capsaicin
title_sort lactobacillus rhamnosus attenuates thai chili extracts induced gut inflammation and dysbiosis despite capsaicin bactericidal effect against the probiotics, a possible toxicity of high dose capsaicin
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8699716/
https://www.ncbi.nlm.nih.gov/pubmed/34941893
http://dx.doi.org/10.1371/journal.pone.0261189
work_keys_str_mv AT panpetchwimonrat lactobacillusrhamnosusattenuatesthaichiliextractsinducedgutinflammationanddysbiosisdespitecapsaicinbactericidaleffectagainsttheprobioticsapossibletoxicityofhighdosecapsaicin
AT visitchanakunpeerapat lactobacillusrhamnosusattenuatesthaichiliextractsinducedgutinflammationanddysbiosisdespitecapsaicinbactericidaleffectagainsttheprobioticsapossibletoxicityofhighdosecapsaicin
AT saisornwilasinee lactobacillusrhamnosusattenuatesthaichiliextractsinducedgutinflammationanddysbiosisdespitecapsaicinbactericidaleffectagainsttheprobioticsapossibletoxicityofhighdosecapsaicin
AT sawatpanichajcharaporn lactobacillusrhamnosusattenuatesthaichiliextractsinducedgutinflammationanddysbiosisdespitecapsaicinbactericidaleffectagainsttheprobioticsapossibletoxicityofhighdosecapsaicin
AT chatthanathonpiraya lactobacillusrhamnosusattenuatesthaichiliextractsinducedgutinflammationanddysbiosisdespitecapsaicinbactericidaleffectagainsttheprobioticsapossibletoxicityofhighdosecapsaicin
AT somboonnanaraporn lactobacillusrhamnosusattenuatesthaichiliextractsinducedgutinflammationanddysbiosisdespitecapsaicinbactericidaleffectagainsttheprobioticsapossibletoxicityofhighdosecapsaicin
AT tumwasornsomying lactobacillusrhamnosusattenuatesthaichiliextractsinducedgutinflammationanddysbiosisdespitecapsaicinbactericidaleffectagainsttheprobioticsapossibletoxicityofhighdosecapsaicin
AT leelahavanichkulasada lactobacillusrhamnosusattenuatesthaichiliextractsinducedgutinflammationanddysbiosisdespitecapsaicinbactericidaleffectagainsttheprobioticsapossibletoxicityofhighdosecapsaicin