Multiplexed CRISPR-mediated engineering of protein secretory pathway genes in the thermotolerant methylotrophic yeast Ogataea thermomethanolica

CRISPR multiplex gRNA systems have been employed in genome engineering in various industrially relevant yeast species. The thermotolerant methylotrophic yeast Ogataea thermomethanolica TBRC 656 is an alternative host for heterologous protein production. However, the limited secretory capability of t...

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Detalles Bibliográficos
Autores principales: Kruasuwan, Worarat, Puseenam, Aekkachai, Tanapongpipat, Sutipa, Roongsawang, Niran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8699913/
https://www.ncbi.nlm.nih.gov/pubmed/34941944
http://dx.doi.org/10.1371/journal.pone.0261754

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8699913/
https://www.ncbi.nlm.nih.gov/pubmed/34941944
http://dx.doi.org/10.1371/journal.pone.0261754

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