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Flow Cytometric Analysis of Bacterial Protein Synthesis: Monitoring Vitality After Water Treatment

Bacterial vitality after water disinfection treatment was investigated using bio-orthogonal non-canonical amino acid tagging (BONCAT) and flow cytometry (FCM). Protein synthesis activity and DNA integrity (BONCAT–SYBR Green) was monitored in Escherichia coli monocultures and in natural marine sample...

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Detalles Bibliográficos
Autores principales: Lindivat, Mathilde, Bratbak, Gunnar, Larsen, Aud, Hess-Erga, Ole-Kristian, Hoell, Ingunn Alne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8702973/
https://www.ncbi.nlm.nih.gov/pubmed/34956134
http://dx.doi.org/10.3389/fmicb.2021.772651