Detection and Quantification of SARS-CoV-2 Receptor Binding Domain Neutralization by a Sensitive Competitive ELISA Assay

This protocol describes an ELISA-based procedure for accurate measurement of SARS-CoV-2 spike protein-receptor binding domain (RBD) neutralization efficacy by murine immune serum. The procedure requires a small amount of S-protein/RBD and angiotensin converting enzyme-2 (ACE2). A high-throughput, si...

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Detalles Bibliográficos
Autores principales: Shalash, Ahmed O., Azuar, Armira, Madge, Harrison Y. R., Modhiran, Naphak, Amarilla, Alberto A., Liang, Benjamin, Khromykh, Alexander A., Watterson, Daniel, Young, Paul R., Toth, Istvan, Skwarczynski, Mariusz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8705285/
https://www.ncbi.nlm.nih.gov/pubmed/34960239
http://dx.doi.org/10.3390/vaccines9121493

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8705285/
https://www.ncbi.nlm.nih.gov/pubmed/34960239
http://dx.doi.org/10.3390/vaccines9121493

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