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Secretome effect of adipose tissue-derived stem cells cultured two-dimensionally and three-dimensionally in mice with streptozocin induced type 1 diabetes
AIMS: To analyze therapeutic potential of the conditioned medium from adipose tissue-derived stem cells (ASC) cultivated in 2D (CM-2D) and 3D (CM-3D) models, in mice with Type 1 diabetes (T1D) induced by streptozotocin. MAIN METHODS: Viability andCD105 expression of 2D and 3D ASC were analyzed by fl...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8710992/ https://www.ncbi.nlm.nih.gov/pubmed/34988430 http://dx.doi.org/10.1016/j.crphar.2021.100069 |
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author | Dias, Isabelle Pinheiro, Daphne Ribeiro Silva, Karina Stumbo, Ana Carolina Thole, Alessandra Cortez, Erika de Carvalho, Laís Carvalho, Simone Nunes |
author_facet | Dias, Isabelle Pinheiro, Daphne Ribeiro Silva, Karina Stumbo, Ana Carolina Thole, Alessandra Cortez, Erika de Carvalho, Laís Carvalho, Simone Nunes |
author_sort | Dias, Isabelle |
collection | PubMed |
description | AIMS: To analyze therapeutic potential of the conditioned medium from adipose tissue-derived stem cells (ASC) cultivated in 2D (CM-2D) and 3D (CM-3D) models, in mice with Type 1 diabetes (T1D) induced by streptozotocin. MAIN METHODS: Viability andCD105 expression of 2D and 3D ASC were analyzed by flow cytometry. T1D was induced in mice by multiple injections of streptozocin. On the 28th and 29th days after the first injection of streptozocin, diabetic animals received CM-2D or CM-3D. Pancreatic, CM-2D, and CM-3D cytokines were analyzed by cytometric bead array (CBA) and insulin and PDX-1 were observed and quantified by immunohistochemistry. Apoptosis-related proteins were quantified by Western Blotting. KEY FINDINGS: ASC in three-dimensional culture released increased levels of IL-6 and IL-2, while IL-4 was decreased. CM-2D induced pancreatic PDX-1 expression and was able to reduce glycemia in diabetic mice one week after injections but not CM-3D. On the other hand, CM-2D and CM-3D were not able to reverse apoptosis of pancreatic cells in diabetic mice nor to increase insulin expression. SIGNIFICANCE: Together, these results demonstrate that the 3D cell culture secretome was not able to improve diabetes type 1 symptoms at the times observed, while 2D cell secretome improved glycemic levels in T1D mice. |
format | Online Article Text |
id | pubmed-8710992 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-87109922022-01-04 Secretome effect of adipose tissue-derived stem cells cultured two-dimensionally and three-dimensionally in mice with streptozocin induced type 1 diabetes Dias, Isabelle Pinheiro, Daphne Ribeiro Silva, Karina Stumbo, Ana Carolina Thole, Alessandra Cortez, Erika de Carvalho, Laís Carvalho, Simone Nunes Curr Res Pharmacol Drug Discov Research Paper AIMS: To analyze therapeutic potential of the conditioned medium from adipose tissue-derived stem cells (ASC) cultivated in 2D (CM-2D) and 3D (CM-3D) models, in mice with Type 1 diabetes (T1D) induced by streptozotocin. MAIN METHODS: Viability andCD105 expression of 2D and 3D ASC were analyzed by flow cytometry. T1D was induced in mice by multiple injections of streptozocin. On the 28th and 29th days after the first injection of streptozocin, diabetic animals received CM-2D or CM-3D. Pancreatic, CM-2D, and CM-3D cytokines were analyzed by cytometric bead array (CBA) and insulin and PDX-1 were observed and quantified by immunohistochemistry. Apoptosis-related proteins were quantified by Western Blotting. KEY FINDINGS: ASC in three-dimensional culture released increased levels of IL-6 and IL-2, while IL-4 was decreased. CM-2D induced pancreatic PDX-1 expression and was able to reduce glycemia in diabetic mice one week after injections but not CM-3D. On the other hand, CM-2D and CM-3D were not able to reverse apoptosis of pancreatic cells in diabetic mice nor to increase insulin expression. SIGNIFICANCE: Together, these results demonstrate that the 3D cell culture secretome was not able to improve diabetes type 1 symptoms at the times observed, while 2D cell secretome improved glycemic levels in T1D mice. Elsevier 2021-11-30 /pmc/articles/PMC8710992/ /pubmed/34988430 http://dx.doi.org/10.1016/j.crphar.2021.100069 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Paper Dias, Isabelle Pinheiro, Daphne Ribeiro Silva, Karina Stumbo, Ana Carolina Thole, Alessandra Cortez, Erika de Carvalho, Laís Carvalho, Simone Nunes Secretome effect of adipose tissue-derived stem cells cultured two-dimensionally and three-dimensionally in mice with streptozocin induced type 1 diabetes |
title | Secretome effect of adipose tissue-derived stem cells cultured two-dimensionally and three-dimensionally in mice with streptozocin induced type 1 diabetes |
title_full | Secretome effect of adipose tissue-derived stem cells cultured two-dimensionally and three-dimensionally in mice with streptozocin induced type 1 diabetes |
title_fullStr | Secretome effect of adipose tissue-derived stem cells cultured two-dimensionally and three-dimensionally in mice with streptozocin induced type 1 diabetes |
title_full_unstemmed | Secretome effect of adipose tissue-derived stem cells cultured two-dimensionally and three-dimensionally in mice with streptozocin induced type 1 diabetes |
title_short | Secretome effect of adipose tissue-derived stem cells cultured two-dimensionally and three-dimensionally in mice with streptozocin induced type 1 diabetes |
title_sort | secretome effect of adipose tissue-derived stem cells cultured two-dimensionally and three-dimensionally in mice with streptozocin induced type 1 diabetes |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8710992/ https://www.ncbi.nlm.nih.gov/pubmed/34988430 http://dx.doi.org/10.1016/j.crphar.2021.100069 |
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