Strategies to Enhance Periplasmic Recombinant Protein Production Yields in Escherichia coli

Main reasons to produce recombinant proteins in the periplasm of E. coli rather than in its cytoplasm are to -i- enable disulfide bond formation, -ii- facilitate protein isolation, -iii- control the nature of the N-terminus of the mature protein, and -iv- minimize exposure to cytoplasmic proteases....

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Detalles Bibliográficos
Autores principales: Karyolaimos, Alexandros, de Gier, Jan-Willem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8712718/
https://www.ncbi.nlm.nih.gov/pubmed/34970535
http://dx.doi.org/10.3389/fbioe.2021.797334