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Strategies to Enhance Periplasmic Recombinant Protein Production Yields in Escherichia coli
Main reasons to produce recombinant proteins in the periplasm of E. coli rather than in its cytoplasm are to -i- enable disulfide bond formation, -ii- facilitate protein isolation, -iii- control the nature of the N-terminus of the mature protein, and -iv- minimize exposure to cytoplasmic proteases....
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Frontiers Media S.A.
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8712718/ https://www.ncbi.nlm.nih.gov/pubmed/34970535 http://dx.doi.org/10.3389/fbioe.2021.797334 |