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A field-deployable method for single and multiplex detection of DNA or RNA from pathogens using Cas12 and Cas13
For some Cas nucleases, trans-cleavage activity triggered by CRISPR/Cas-mediated cis-cleavage upon target nucleic acid recognition has been explored for diagnostic detection. Portable single and multiplex nucleic acid-based detection is needed for crop pathogen management in agriculture. Here, we ha...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Science China Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8713540/ https://www.ncbi.nlm.nih.gov/pubmed/34962615 http://dx.doi.org/10.1007/s11427-021-2028-x |