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A field-deployable method for single and multiplex detection of DNA or RNA from pathogens using Cas12 and Cas13

For some Cas nucleases, trans-cleavage activity triggered by CRISPR/Cas-mediated cis-cleavage upon target nucleic acid recognition has been explored for diagnostic detection. Portable single and multiplex nucleic acid-based detection is needed for crop pathogen management in agriculture. Here, we ha...

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Detalles Bibliográficos
Autores principales: Li, Lina, Duan, Canxing, Weng, Jianfeng, Qi, Xiantao, Liu, Changlin, Li, Xinhai, Zhu, Jinjie, Xie, Chuanxiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Science China Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8713540/
https://www.ncbi.nlm.nih.gov/pubmed/34962615
http://dx.doi.org/10.1007/s11427-021-2028-x