A fast, aqueous, reversible three-day tissue clearing method for adult and embryonic mouse brain and whole body

Optical clearing methods serve as powerful tools to study intact organs and neuronal circuits. We developed an aqueous clearing protocol, Fast 3D Clear, that relies on tetrahydrofuran for tissue delipidation and iohexol for clearing, such that tissues can be imaged under immersion oil in light-sheet...

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Detalles Bibliográficos
Autores principales: Kosmidis, Stylianos, Negrean, Adrian, Dranovsky, Alex, Losonczy, Attila, Kandel, Eric R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8713566/
https://www.ncbi.nlm.nih.gov/pubmed/34966901
http://dx.doi.org/10.1016/j.crmeth.2021.100090

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8713566/
https://www.ncbi.nlm.nih.gov/pubmed/34966901
http://dx.doi.org/10.1016/j.crmeth.2021.100090

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