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A fast, aqueous, reversible three-day tissue clearing method for adult and embryonic mouse brain and whole body
Optical clearing methods serve as powerful tools to study intact organs and neuronal circuits. We developed an aqueous clearing protocol, Fast 3D Clear, that relies on tetrahydrofuran for tissue delipidation and iohexol for clearing, such that tissues can be imaged under immersion oil in light-sheet...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8713566/ https://www.ncbi.nlm.nih.gov/pubmed/34966901 http://dx.doi.org/10.1016/j.crmeth.2021.100090 |