Optimized protocols for efficient gene editing in mouse hepatocytes in vivo using CRISPR-Cas9 technology

We provide a protocol for gene editing in mouse hepatocytes in vivo using the CRISPR-Cas9 technology via AAV delivery. This protocol describes the construction of AAV plasmids, AAV packaging, injection, and the detection of in vivo knockout efficiency. Using this protocol, we can get up to 10(14) AA...

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Detalles Bibliográficos
Autores principales: Chen, Yanhao, Ding, Qiurong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8715323/
https://www.ncbi.nlm.nih.gov/pubmed/35005644
http://dx.doi.org/10.1016/j.xpro.2021.101062

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8715323/
https://www.ncbi.nlm.nih.gov/pubmed/35005644
http://dx.doi.org/10.1016/j.xpro.2021.101062

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