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Optimized protocols for efficient gene editing in mouse hepatocytes in vivo using CRISPR-Cas9 technology
We provide a protocol for gene editing in mouse hepatocytes in vivo using the CRISPR-Cas9 technology via AAV delivery. This protocol describes the construction of AAV plasmids, AAV packaging, injection, and the detection of in vivo knockout efficiency. Using this protocol, we can get up to 10(14) AA...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8715323/ https://www.ncbi.nlm.nih.gov/pubmed/35005644 http://dx.doi.org/10.1016/j.xpro.2021.101062 |