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Optimized CRISPR-RfxCas13d system for RNA targeting in zebrafish embryos
CRISPR-Cas systems have been used to induce DNA mutagenesis for gene function discovery. However, the development of tools to eliminate RNAs provides complementary and unique approaches to disrupt gene expression. Here, we present a workflow to perform specific, efficient, and cost-effective mRNA kn...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8715325/ https://www.ncbi.nlm.nih.gov/pubmed/35005640 http://dx.doi.org/10.1016/j.xpro.2021.101058 |