Optimized CRISPR-RfxCas13d system for RNA targeting in zebrafish embryos

CRISPR-Cas systems have been used to induce DNA mutagenesis for gene function discovery. However, the development of tools to eliminate RNAs provides complementary and unique approaches to disrupt gene expression. Here, we present a workflow to perform specific, efficient, and cost-effective mRNA kn...

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Detalles Bibliográficos
Autores principales: Hernandez-Huertas, Luis, Kushawah, Gopal, Diaz-Moscoso, Alejandro, Tomas-Gallardo, Laura, Moreno-Sanchez, Ismael, da Silva Pescador, Gabriel, Bazzini, Ariel A., Moreno-Mateos, Miguel A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8715325/
https://www.ncbi.nlm.nih.gov/pubmed/35005640
http://dx.doi.org/10.1016/j.xpro.2021.101058

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