A deletion in the N gene of SARS-CoV-2 may reduce test sensitivity for detection of SARS-CoV-2

One SARS-CoV-2-positive sample demonstrated impaired detection of the N1 target by RT-PCR using US CDC primer/probe sets. A 3 nucleotide deletion was discovered that overlaps the forward primer binding site. This finding underscores the importance of continued SARS-CoV-2 mutation surveillance and as...

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Autores principales: Wang, Huanyu, Jean, Sophonie, Wilson, Sarah A., Lucyshyn, Jocelyn M., McGrath, Sean, Wilson, Richard K., Magrini, Vincent, Leber, Amy L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8715644/
https://www.ncbi.nlm.nih.gov/pubmed/35045382
http://dx.doi.org/10.1016/j.diagmicrobio.2021.115631
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author Wang, Huanyu
Jean, Sophonie
Wilson, Sarah A.
Lucyshyn, Jocelyn M.
McGrath, Sean
Wilson, Richard K.
Magrini, Vincent
Leber, Amy L.
author_facet Wang, Huanyu
Jean, Sophonie
Wilson, Sarah A.
Lucyshyn, Jocelyn M.
McGrath, Sean
Wilson, Richard K.
Magrini, Vincent
Leber, Amy L.
author_sort Wang, Huanyu
collection PubMed
description One SARS-CoV-2-positive sample demonstrated impaired detection of the N1 target by RT-PCR using US CDC primer/probe sets. A 3 nucleotide deletion was discovered that overlaps the forward primer binding site. This finding underscores the importance of continued SARS-CoV-2 mutation surveillance and assessment of the impact on diagnostic test performance.
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spelling pubmed-87156442021-12-29 A deletion in the N gene of SARS-CoV-2 may reduce test sensitivity for detection of SARS-CoV-2 Wang, Huanyu Jean, Sophonie Wilson, Sarah A. Lucyshyn, Jocelyn M. McGrath, Sean Wilson, Richard K. Magrini, Vincent Leber, Amy L. Diagn Microbiol Infect Dis Article One SARS-CoV-2-positive sample demonstrated impaired detection of the N1 target by RT-PCR using US CDC primer/probe sets. A 3 nucleotide deletion was discovered that overlaps the forward primer binding site. This finding underscores the importance of continued SARS-CoV-2 mutation surveillance and assessment of the impact on diagnostic test performance. Elsevier Inc. 2022-04 2021-12-29 /pmc/articles/PMC8715644/ /pubmed/35045382 http://dx.doi.org/10.1016/j.diagmicrobio.2021.115631 Text en © 2021 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Wang, Huanyu
Jean, Sophonie
Wilson, Sarah A.
Lucyshyn, Jocelyn M.
McGrath, Sean
Wilson, Richard K.
Magrini, Vincent
Leber, Amy L.
A deletion in the N gene of SARS-CoV-2 may reduce test sensitivity for detection of SARS-CoV-2
title A deletion in the N gene of SARS-CoV-2 may reduce test sensitivity for detection of SARS-CoV-2
title_full A deletion in the N gene of SARS-CoV-2 may reduce test sensitivity for detection of SARS-CoV-2
title_fullStr A deletion in the N gene of SARS-CoV-2 may reduce test sensitivity for detection of SARS-CoV-2
title_full_unstemmed A deletion in the N gene of SARS-CoV-2 may reduce test sensitivity for detection of SARS-CoV-2
title_short A deletion in the N gene of SARS-CoV-2 may reduce test sensitivity for detection of SARS-CoV-2
title_sort deletion in the n gene of sars-cov-2 may reduce test sensitivity for detection of sars-cov-2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8715644/
https://www.ncbi.nlm.nih.gov/pubmed/35045382
http://dx.doi.org/10.1016/j.diagmicrobio.2021.115631
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