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A deletion in the N gene of SARS-CoV-2 may reduce test sensitivity for detection of SARS-CoV-2
One SARS-CoV-2-positive sample demonstrated impaired detection of the N1 target by RT-PCR using US CDC primer/probe sets. A 3 nucleotide deletion was discovered that overlaps the forward primer binding site. This finding underscores the importance of continued SARS-CoV-2 mutation surveillance and as...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8715644/ https://www.ncbi.nlm.nih.gov/pubmed/35045382 http://dx.doi.org/10.1016/j.diagmicrobio.2021.115631 |
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author | Wang, Huanyu Jean, Sophonie Wilson, Sarah A. Lucyshyn, Jocelyn M. McGrath, Sean Wilson, Richard K. Magrini, Vincent Leber, Amy L. |
author_facet | Wang, Huanyu Jean, Sophonie Wilson, Sarah A. Lucyshyn, Jocelyn M. McGrath, Sean Wilson, Richard K. Magrini, Vincent Leber, Amy L. |
author_sort | Wang, Huanyu |
collection | PubMed |
description | One SARS-CoV-2-positive sample demonstrated impaired detection of the N1 target by RT-PCR using US CDC primer/probe sets. A 3 nucleotide deletion was discovered that overlaps the forward primer binding site. This finding underscores the importance of continued SARS-CoV-2 mutation surveillance and assessment of the impact on diagnostic test performance. |
format | Online Article Text |
id | pubmed-8715644 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-87156442021-12-29 A deletion in the N gene of SARS-CoV-2 may reduce test sensitivity for detection of SARS-CoV-2 Wang, Huanyu Jean, Sophonie Wilson, Sarah A. Lucyshyn, Jocelyn M. McGrath, Sean Wilson, Richard K. Magrini, Vincent Leber, Amy L. Diagn Microbiol Infect Dis Article One SARS-CoV-2-positive sample demonstrated impaired detection of the N1 target by RT-PCR using US CDC primer/probe sets. A 3 nucleotide deletion was discovered that overlaps the forward primer binding site. This finding underscores the importance of continued SARS-CoV-2 mutation surveillance and assessment of the impact on diagnostic test performance. Elsevier Inc. 2022-04 2021-12-29 /pmc/articles/PMC8715644/ /pubmed/35045382 http://dx.doi.org/10.1016/j.diagmicrobio.2021.115631 Text en © 2021 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Wang, Huanyu Jean, Sophonie Wilson, Sarah A. Lucyshyn, Jocelyn M. McGrath, Sean Wilson, Richard K. Magrini, Vincent Leber, Amy L. A deletion in the N gene of SARS-CoV-2 may reduce test sensitivity for detection of SARS-CoV-2 |
title | A deletion in the N gene of SARS-CoV-2 may reduce test sensitivity for detection of SARS-CoV-2 |
title_full | A deletion in the N gene of SARS-CoV-2 may reduce test sensitivity for detection of SARS-CoV-2 |
title_fullStr | A deletion in the N gene of SARS-CoV-2 may reduce test sensitivity for detection of SARS-CoV-2 |
title_full_unstemmed | A deletion in the N gene of SARS-CoV-2 may reduce test sensitivity for detection of SARS-CoV-2 |
title_short | A deletion in the N gene of SARS-CoV-2 may reduce test sensitivity for detection of SARS-CoV-2 |
title_sort | deletion in the n gene of sars-cov-2 may reduce test sensitivity for detection of sars-cov-2 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8715644/ https://www.ncbi.nlm.nih.gov/pubmed/35045382 http://dx.doi.org/10.1016/j.diagmicrobio.2021.115631 |
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