CRISPR-Cas12a ribonucleoprotein-mediated gene editing in the plant pathogenic fungus Magnaporthe oryzae

Gene replacements through homologous recombination (HR) have been extensively used for functional genomic studies. However, the general efficiency of HR repair can be low in filamentous fungi and the process laborious. Here, we provide a detailed protocol for efficient gene editing by inserting dono...

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Detalles Bibliográficos
Autores principales: Huang, Jun, Cook, David E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8717591/
https://www.ncbi.nlm.nih.gov/pubmed/35005648
http://dx.doi.org/10.1016/j.xpro.2021.101072

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8717591/
https://www.ncbi.nlm.nih.gov/pubmed/35005648
http://dx.doi.org/10.1016/j.xpro.2021.101072

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