Analyzing mouse neural stem cell and progenitor cell proliferation using EdU incorporation and multicolor flow cytometry

This protocol describes an ex vivo approach to identify and quantify the proportions of proliferating neural stem cells and progenitors of the mouse subventricular zone. It uses ethynyl deoxyuridine (EdU) incorporation to identify dividing cells, combined with multicolor flow cytometry for 4 cell su...

Descripción completa

Detalles Bibliográficos
Autores principales: Velloso, Fernando Janczur, Kumari, Ekta, Buono, Krista D., Frondelli, Michelle J., Levison, Steven W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718722/
https://www.ncbi.nlm.nih.gov/pubmed/35005647
http://dx.doi.org/10.1016/j.xpro.2021.101065

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718722/
https://www.ncbi.nlm.nih.gov/pubmed/35005647
http://dx.doi.org/10.1016/j.xpro.2021.101065

Ejemplares similares