Analyzing mouse neural stem cell and progenitor cell proliferation using EdU incorporation and multicolor flow cytometry

This protocol describes an ex vivo approach to identify and quantify the proportions of proliferating neural stem cells and progenitors of the mouse subventricular zone. It uses ethynyl deoxyuridine (EdU) incorporation to identify dividing cells, combined with multicolor flow cytometry for 4 cell su...

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Detalles Bibliográficos
Autores principales: Velloso, Fernando Janczur, Kumari, Ekta, Buono, Krista D., Frondelli, Michelle J., Levison, Steven W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718722/
https://www.ncbi.nlm.nih.gov/pubmed/35005647
http://dx.doi.org/10.1016/j.xpro.2021.101065
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author Velloso, Fernando Janczur
Kumari, Ekta
Buono, Krista D.
Frondelli, Michelle J.
Levison, Steven W.
author_facet Velloso, Fernando Janczur
Kumari, Ekta
Buono, Krista D.
Frondelli, Michelle J.
Levison, Steven W.
author_sort Velloso, Fernando Janczur
collection PubMed
description This protocol describes an ex vivo approach to identify and quantify the proportions of proliferating neural stem cells and progenitors of the mouse subventricular zone. It uses ethynyl deoxyuridine (EdU) incorporation to identify dividing cells, combined with multicolor flow cytometry for 4 cell surface antigens to distinguish between 8 phenotypically distinct mouse neural progenitors and stem cells. It has been optimized for wild-type neonatal mice but can be used on mice of any postnatal age. For complete details on the use and execution of this profile, please refer to Kumari et al. (2020).
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spelling pubmed-87187222022-01-06 Analyzing mouse neural stem cell and progenitor cell proliferation using EdU incorporation and multicolor flow cytometry Velloso, Fernando Janczur Kumari, Ekta Buono, Krista D. Frondelli, Michelle J. Levison, Steven W. STAR Protoc Protocol This protocol describes an ex vivo approach to identify and quantify the proportions of proliferating neural stem cells and progenitors of the mouse subventricular zone. It uses ethynyl deoxyuridine (EdU) incorporation to identify dividing cells, combined with multicolor flow cytometry for 4 cell surface antigens to distinguish between 8 phenotypically distinct mouse neural progenitors and stem cells. It has been optimized for wild-type neonatal mice but can be used on mice of any postnatal age. For complete details on the use and execution of this profile, please refer to Kumari et al. (2020). Elsevier 2021-12-23 /pmc/articles/PMC8718722/ /pubmed/35005647 http://dx.doi.org/10.1016/j.xpro.2021.101065 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Velloso, Fernando Janczur
Kumari, Ekta
Buono, Krista D.
Frondelli, Michelle J.
Levison, Steven W.
Analyzing mouse neural stem cell and progenitor cell proliferation using EdU incorporation and multicolor flow cytometry
title Analyzing mouse neural stem cell and progenitor cell proliferation using EdU incorporation and multicolor flow cytometry
title_full Analyzing mouse neural stem cell and progenitor cell proliferation using EdU incorporation and multicolor flow cytometry
title_fullStr Analyzing mouse neural stem cell and progenitor cell proliferation using EdU incorporation and multicolor flow cytometry
title_full_unstemmed Analyzing mouse neural stem cell and progenitor cell proliferation using EdU incorporation and multicolor flow cytometry
title_short Analyzing mouse neural stem cell and progenitor cell proliferation using EdU incorporation and multicolor flow cytometry
title_sort analyzing mouse neural stem cell and progenitor cell proliferation using edu incorporation and multicolor flow cytometry
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718722/
https://www.ncbi.nlm.nih.gov/pubmed/35005647
http://dx.doi.org/10.1016/j.xpro.2021.101065
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