Cargando…

Analyzing mouse neural stem cell and progenitor cell proliferation using EdU incorporation and multicolor flow cytometry

This protocol describes an ex vivo approach to identify and quantify the proportions of proliferating neural stem cells and progenitors of the mouse subventricular zone. It uses ethynyl deoxyuridine (EdU) incorporation to identify dividing cells, combined with multicolor flow cytometry for 4 cell su...

Descripción completa

Detalles Bibliográficos
Autores principales:	Velloso, Fernando Janczur, Kumari, Ekta, Buono, Krista D., Frondelli, Michelle J., Levison, Steven W.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718722/ https://www.ncbi.nlm.nih.gov/pubmed/35005647 http://dx.doi.org/10.1016/j.xpro.2021.101065

Ejemplares similares

An EdU-based flow cytometry assay to evaluate chicken T lymphocyte proliferation
por: Alvarez, Karla Lucía F., et al.
Publicado: (2020)

Developmental IL-6 Exposure Favors Production of PDGF-Responsive Multipotential Progenitors at the Expense of Neural Stem Cells and Other Progenitors
por: Kumari, Ekta, et al.
Publicado: (2020)

Measuring S-Phase Duration from Asynchronous Cells Using Dual EdU-BrdU Pulse-Chase Labeling Flow Cytometry
por: Bialic, Marta, et al.
Publicado: (2022)

Three Optimized Methods for In Situ Quantification of Progenitor Cell Proliferation in Embryonic Kidneys Using BrdU, EdU, and PCNA
por: O’Hara, Rosalie E., et al.
Publicado: (2019)

Visualization of Mitochondrial DNA Replication in Individual Cells by EdU Signal Amplification
por: Haines, Kristine M., et al.
Publicado: (2010)

EdU induces DNA damage response and cell death in mESC in culture
por: Kohlmeier, Fanni, et al.
Publicado: (2013)

A rapid non-radioactive technique for measurement of repair synthesis in primary human fibroblasts by incorporation of ethynyl deoxyuridine (EdU)
por: Limsirichaikul, Siripan, et al.
Publicado: (2009)

A modified method to analyse cell proliferation using EdU labelling in large insect brains
por: Anton, Amaia Alcalde, et al.
Publicado: (2023)

Leukemia Inhibitory Factor Is Required for Subventricular Zone Astrocyte Progenitor Proliferation and for Prokineticin-2 Production after a Closed Head Injury in Mice
por: Frondelli, Michelle J., et al.
Publicado: (2021)

In Situ Labeling of Mitochondrial DNA Replication in Drosophila Adult Ovaries by EdU Staining
por: Chen, Zhe, et al.
Publicado: (2016)

Single-sister labeling in the C. elegans germline using the nucleotide analog EdU
por: Almanzar, David E., et al.
Publicado: (2022)

Quantification of cell cycle kinetics by EdU (5-ethynyl-2′-deoxyuridine)-coupled-fluorescence-intensity analysis
por: Pereira, Pedro D., et al.
Publicado: (2017)

Neural Stem Cells in Adult Mammals are not Astrocytes
por: Velloso, Fernando Janczur, et al.
Publicado: (2022)

Actively replicating gut bacteria identified by 5-ethynyl-2’-deoxyuridine (EdU) click chemistry and cell sorting
por: Beauchemin, Eve T., et al.
Publicado: (2023)

Quantitative Evaluation of in Vivo Target Efficacy of Anti-tumor Agents via an Immunofluorescence and EdU Labeling Strategy
por: He, Yujun, et al.
Publicado: (2018)

EdU-Based Step-by-Step Method for the Detection of Sister Chromatid Exchanges for Application in Plant Genotoxicity Assessment
por: Kwasniewska, Jolanta, et al.
Publicado: (2020)

Unmasking the responses of the stem cells and progenitors in the subventricular zone after neonatal and pediatric brain injuries
por: Clausi, Mariano Guardia, et al.
Publicado: (2016)

A Simple Method to Assess In Vivo Proliferation in Lung Vasculature with EdU: The Case of MMC-Induced PVOD in Rat
por: Fabrice, Antigny, et al.
Publicado: (2015)

Corrigendum to “A Simple Method to Assess In Vivo Proliferation in Lung Vasculature with EdU: The Case of MMC-Induced PVOD in Rat”
por: Antigny, Fabrice, et al.
Publicado: (2017)

X-autosome and X-Y Translocations in Female Carriers: X-chromosome Inactivation Easily Detectable by 5-ethynyl-2-deoxyuridine (EdU)
por: Donat, M, et al.
Publicado: (2017)

Comparison of an image cytometry somatic cell count analyzer to a flow cytometry analyzer
por: Hubner, A., et al.
Publicado: (2023)

Multi-set Pre-processing of Multicolor Flow Cytometry Data
por: Folcarelli, Rita, et al.
Publicado: (2020)

Multicolor flow cytometry in clinical samples for platelet signaling assessment
por: Garcia, Cedric, et al.
Publicado: (2023)

CERNland: addicted to edu-gaming
por: Antonella Del Rosso
Publicado: (2013)

A novel direct co-culture assay analyzed by multicolor flow cytometry reveals context- and cell type-specific immunomodulatory effects of equine mesenchymal stromal cells
por: Hillmann, Aline, et al.
Publicado: (2019)

Edu-gestión : proyecto "Modelo educativo G-PEUX 2002"

Detection of clinically relevant immune checkpoint markers by multicolor flow cytometry
por: Cunningham, Rachel A., et al.
Publicado: (2019)

A novel multicolor flow-cytometry application for quantitative detection of receptors on vascular smooth muscle cells
por: Radziwon-Balicka, Aneta, et al.
Publicado: (2017)

Multicolor flow cytometry immunophenotyping and characterization of aneuploidy in pediatric B-cell precursor acute lymphoblastic leukemia
por: Pierzyna-Świtała, Magdalena, et al.
Publicado: (2021)

Pediatric ED Saves: Analyzing the ED Screen of Direct Admissions
por: Ramirez-Cueva, Fatima, et al.
Publicado: (2023)

Improving Multicolor Colocalization in Single-Vesicle Flow Cytometry with Vesicle Transit Time
por: Andronico, Luca A., et al.
Publicado: (2023)

Spectral Cytometry Has Unique Properties Allowing Multicolor Analysis of Cell Suspensions Isolated from Solid Tissues
por: Schmutz, Sandrine, et al.
Publicado: (2016)

Validation of an automated technique for ovarian cortex dissociation: isolation of viable ovarian cells and their qualification by multicolor flow cytometry
por: Zver, Tristan, et al.
Publicado: (2017)

Multicolor Flow Cytometry and Cytokine Analysis Provides Enhanced Information on Kidney Transplant Biopsies
por: Muczynski, Kimberly A., et al.
Publicado: (2018)

Isolating and Analyzing Cells of the Pancreas Mesenchyme by Flow Cytometry
por: Epshtein, Alona, et al.
Publicado: (2017)

eduSPIM: Light Sheet Microscopy in the Museum
por: Jahr, Wiebke, et al.
Publicado: (2016)

Distribution of major lymphocyte subsets and memory T-cell subpopulations in healthy adults employing GLP-conforming multicolor flow cytometry
por: Schultze-Florey, Christian R., et al.
Publicado: (2021)

Dynamic evaluation of blood immune cells predictive of response to immune checkpoint inhibitors in NSCLC by multicolor spectrum flow cytometry
por: Ma, Weijie, et al.
Publicado: (2023)

Development of Multicolor Flow Cytometry Calibration Standards: Assignment of Equivalent Reference Fluorophores (ERF) Unit
por: Wang, Lili, et al.
Publicado: (2011)

Label-free chemical imaging flow cytometry by high-speed multicolor stimulated Raman scattering
por: Suzuki, Yuta, et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_l768ikdlr07r462h737idakt8r