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High cell density cultivation of E. coli in shake flasks for the production of recombinant proteins
Batch cultivation of recombinant bacteria in shake flasks typically results in low cell density due to nutrient depletion. Previous studies on high cell density cultivation in shake flasks have relied mainly on controlled release mechanisms. Here, we report a true fed-batch strategy to achieve high...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718739/ https://www.ncbi.nlm.nih.gov/pubmed/35004235 http://dx.doi.org/10.1016/j.btre.2021.e00694 |