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High cell density cultivation of E. coli in shake flasks for the production of recombinant proteins

Batch cultivation of recombinant bacteria in shake flasks typically results in low cell density due to nutrient depletion. Previous studies on high cell density cultivation in shake flasks have relied mainly on controlled release mechanisms. Here, we report a true fed-batch strategy to achieve high...

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Detalles Bibliográficos
Autores principales: Ganjave, Snehal D, Dodia, Hardik, Sunder, Avinash Vellore, Madhu, Swati, Wangikar, Pramod P
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718739/
https://www.ncbi.nlm.nih.gov/pubmed/35004235
http://dx.doi.org/10.1016/j.btre.2021.e00694