Cargando…

Heterogeneity of human anti-viral immunity shaped by virus, tissue, age, and sex

The persistence of anti-viral immunity is essential for protection and exhibits profound heterogeneity across individuals. Here, we elucidate the factors that shape maintenance and function of anti-viral T cell immunity in the body by comprehensive profiling of virus-specific T cells across blood, l...

Descripción completa

Detalles Bibliográficos
Autores principales: Poon, Maya M.L., Byington, Eve, Meng, Wenzhao, Kubota, Masaru, Matsumoto, Rei, Grifoni, Alba, Weiskopf, Daniela, Dogra, Pranay, Lam, Nora, Szabo, Peter A., Ural, Basak Burcu, Wells, Steven B., Rosenfeld, Aaron M., Brusko, Maigan A., Brusko, Todd M., Connors, Thomas J., Sette, Alessandro, Sims, Peter A., Prak, Eline T. Luning, Shen, Yufeng, Farber, Donna L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8719595/
https://www.ncbi.nlm.nih.gov/pubmed/34852222
http://dx.doi.org/10.1016/j.celrep.2021.110071
Descripción
Sumario:The persistence of anti-viral immunity is essential for protection and exhibits profound heterogeneity across individuals. Here, we elucidate the factors that shape maintenance and function of anti-viral T cell immunity in the body by comprehensive profiling of virus-specific T cells across blood, lymphoid organs, and mucosal tissues of organ donors. We use flow cytometry, T cell receptor sequencing, single-cell transcriptomics, and cytokine analysis to profile virus-specific CD8(+) T cells recognizing the ubiquitous pathogens influenza and cytomegalovirus. Our results reveal that virus specificity determines overall magnitude, tissue distribution, differentiation, and clonal repertoire of virus-specific T cells. Age and sex influence T cell differentiation and dissemination in tissues, while T cell tissue residence and functionality are highly correlated with the site. Together, our results demonstrate how the covariates of virus, tissue, age, and sex impact the anti-viral immune response, which is important for targeting, monitoring, and predicting immune responses to existing and emerging viruses.