Media optimization for SHuffle T7 Escherichia coli expressing SUMO-Lispro proinsulin by response surface methodology

BACKGROUND: SHuffle is a suitable Escherichia coli (E. coli) strain for high yield cytoplasmic soluble expression of disulfide-bonded proteins such as Insulin due to its oxidative cytoplasmic condition and the ability to correct the arrangement of disulfide bonds. Lispro is an Insulin analog that is...

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Detalles Bibliográficos
Autores principales: Khalilvand, Aida Bakhshi, Aminzadeh, Saeed, Sanati, Mohammad Hossein, Mahboudi, Fereidoun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8722112/
https://www.ncbi.nlm.nih.gov/pubmed/34980082
http://dx.doi.org/10.1186/s12896-021-00732-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8722112/
https://www.ncbi.nlm.nih.gov/pubmed/34980082
http://dx.doi.org/10.1186/s12896-021-00732-4

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