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A case for glycerol as an acceptable additive for single-particle cryoEM samples

Buffer-composition and sample-preparation guidelines for cryo-electron microscopy are geared towards maximizing imaging contrast and reducing electron-beam-induced motion. These pursuits often involve the minimization or the complete removal of additives that are commonly used to facilitate proper p...

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Detalles Bibliográficos
Autores principales: Basanta, Benjamin, Hirschi, Marscha M., Grotjahn, Danielle A., Lander, Gabriel C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8725161/
https://www.ncbi.nlm.nih.gov/pubmed/34981768
http://dx.doi.org/10.1107/S2059798321012110