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Construction of a CRISPR/Cas9-Mediated Genome Editing System in Lentinula edodes

CRISPR/Cas9 genome editing systems have been established in a broad range of eukaryotic species. Herein, we report the first method for genetic engineering in pyogo (shiitake) mushrooms (Lentinula edodes) using CRISPR/Cas9. For in vivo expression of guide RNAs (gRNAs) targeting the mating-type gene...

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Detalles Bibliográficos
Autores principales: Moon, Suyun, An, Jee Young, Choi, Yeon-Jae, Oh, Youn-Lee, Ro, Hyeon-Su, Ryu, Hojin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8725921/
https://www.ncbi.nlm.nih.gov/pubmed/35035251
http://dx.doi.org/10.1080/12298093.2021.2006401