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Optimized two‐step electroporation process to achieve efficient nonviral‐mediated gene insertion into primary T cells
The development of gene editing technologies over the past years has allowed the precise and efficient insertion of transgenes into the genome of various cell types. Knock‐in approaches using homology‐directed repair and designer nucleases often rely on viral vectors, which can considerably impact t...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8727936/ https://www.ncbi.nlm.nih.gov/pubmed/34510816 http://dx.doi.org/10.1002/2211-5463.13292 |