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A single-molecule localization microscopy method for tissues reveals nonrandom nuclear pore distribution in Drosophila
Single-molecule localization microscopy (SMLM) can provide nanoscale resolution in thin samples but has rarely been applied to tissues because of high background from out-of-focus emitters and optical aberrations. Here, we describe a line scanning microscope that provides optical sectioning for SMLM...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8729783/ https://www.ncbi.nlm.nih.gov/pubmed/34806753 http://dx.doi.org/10.1242/jcs.259570 |