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A single-molecule localization microscopy method for tissues reveals nonrandom nuclear pore distribution in Drosophila

Single-molecule localization microscopy (SMLM) can provide nanoscale resolution in thin samples but has rarely been applied to tissues because of high background from out-of-focus emitters and optical aberrations. Here, we describe a line scanning microscope that provides optical sectioning for SMLM...

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Detalles Bibliográficos
Autores principales: Cheng, Jinmei, Allgeyer, Edward S., Richens, Jennifer H., Dzafic, Edo, Palandri, Amandine, Lewków, Bohdan, Sirinakis, George, St Johnston, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8729783/
https://www.ncbi.nlm.nih.gov/pubmed/34806753
http://dx.doi.org/10.1242/jcs.259570