High-throughput and high-content bioassay enables tuning of polyester nanoparticles for cellular uptake, endosomal escape, and systemic in vivo delivery of mRNA

Nanoparticle-based mRNA therapeutics hold great promise, but cellular internalization and endosomal escape remain key barriers for cytosolic delivery. We developed a dual nanoparticle uptake and endosomal disruption assay using high-throughput and high-content image-based screening. Using a genetica...

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Detalles Bibliográficos
Autores principales: Rui, Yuan, Wilson, David R., Tzeng, Stephany Y., Yamagata, Hannah M., Sudhakar, Deepti, Conge, Marranne, Berlinicke, Cynthia A., Zack, Donald J., Tuesca, Anthony, Green, Jordan J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2022
Materias:
Biomedicine and Life Sciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8730632/
https://www.ncbi.nlm.nih.gov/pubmed/34985952
http://dx.doi.org/10.1126/sciadv.abk2855

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8730632/
https://www.ncbi.nlm.nih.gov/pubmed/34985952
http://dx.doi.org/10.1126/sciadv.abk2855

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