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High-throughput and high-content bioassay enables tuning of polyester nanoparticles for cellular uptake, endosomal escape, and systemic in vivo delivery of mRNA

Nanoparticle-based mRNA therapeutics hold great promise, but cellular internalization and endosomal escape remain key barriers for cytosolic delivery. We developed a dual nanoparticle uptake and endosomal disruption assay using high-throughput and high-content image-based screening. Using a genetica...

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Detalles Bibliográficos
Autores principales:	Rui, Yuan, Wilson, David R., Tzeng, Stephany Y., Yamagata, Hannah M., Sudhakar, Deepti, Conge, Marranne, Berlinicke, Cynthia A., Zack, Donald J., Tuesca, Anthony, Green, Jordan J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Association for the Advancement of Science 2022
Materias:	Biomedicine and Life Sciences
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8730632/ https://www.ncbi.nlm.nih.gov/pubmed/34985952 http://dx.doi.org/10.1126/sciadv.abk2855

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