Single nucleotide substitutions effectively block Cas9 and allow for scarless genome editing in Caenorhabditis elegans

In Caenorhabditis elegans, germline injection of Cas9 complexes is reliably used to achieve genome editing through homology-directed repair of Cas9-generated DNA breaks. To prevent Cas9 from targeting repaired DNA, additional blocking mutations are often incorporated into homologous repair templates...

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Detalles Bibliográficos
Autores principales: Medley, Jeffrey C, Hebbar, Shilpa, Sydzyik, Joel T, Zinovyeva, Anna Y
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Investigation
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8733430/
https://www.ncbi.nlm.nih.gov/pubmed/34791245
http://dx.doi.org/10.1093/genetics/iyab199

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8733430/
https://www.ncbi.nlm.nih.gov/pubmed/34791245
http://dx.doi.org/10.1093/genetics/iyab199

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