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Single nucleotide substitutions effectively block Cas9 and allow for scarless genome editing in Caenorhabditis elegans
In Caenorhabditis elegans, germline injection of Cas9 complexes is reliably used to achieve genome editing through homology-directed repair of Cas9-generated DNA breaks. To prevent Cas9 from targeting repaired DNA, additional blocking mutations are often incorporated into homologous repair templates...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8733430/ https://www.ncbi.nlm.nih.gov/pubmed/34791245 http://dx.doi.org/10.1093/genetics/iyab199 |