Evaluating the feasibility of Cas9 overexpression in 3T3-L1 cells for generation of genetic knock-out adipocyte cell lines

Cell lines recapitulating physiological processes can represent alternatives to animal or human studies. The 3T3-L1 cell line is used to mimic adipocyte function and differentiation. Since transfection of 3T3-L1 cells is difficult, we used a modified 3T3-L1 cell line overexpressing Cas9 for a straig...

Descripción completa

Detalles Bibliográficos
Autores principales: Suchý, Tomás, Kaczmarek, Isabell, Maricic, Tomislav, Zieschang, Christian, Schöneberg, Torsten, Thor, Doreen, Liebscher, Ines
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Technical Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8735834/
https://www.ncbi.nlm.nih.gov/pubmed/34915813
http://dx.doi.org/10.1080/21623945.2021.1990480

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8735834/
https://www.ncbi.nlm.nih.gov/pubmed/34915813
http://dx.doi.org/10.1080/21623945.2021.1990480

Ejemplares similares