Evaluation of Subchronic Toxicity and Genotoxicity of Ethanolic Extract of Aster glehni Leaves and Stems

Aster glehni, a traditional plant on Ulleung Island in the Republic of Korea, has been recognized for its multiple medicinal properties. However, potential toxicity and safety analyses of A. glehni have not been previously investigated. Therefore, this study aimed to evaluate the safety profile of e...

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Autores principales: Lim, Mi Kyung, Kim, Ju Yeon, Jeong, Jeongho, Han, Eun Hye, Lee, Sang Ho, Lee, Soyeon, Kim, Sun-Don, Lee, Jinu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8736694/
https://www.ncbi.nlm.nih.gov/pubmed/35003288
http://dx.doi.org/10.1155/2021/1018101
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author Lim, Mi Kyung
Kim, Ju Yeon
Jeong, Jeongho
Han, Eun Hye
Lee, Sang Ho
Lee, Soyeon
Kim, Sun-Don
Lee, Jinu
author_facet Lim, Mi Kyung
Kim, Ju Yeon
Jeong, Jeongho
Han, Eun Hye
Lee, Sang Ho
Lee, Soyeon
Kim, Sun-Don
Lee, Jinu
author_sort Lim, Mi Kyung
collection PubMed
description Aster glehni, a traditional plant on Ulleung Island in the Republic of Korea, has been recognized for its multiple medicinal properties. However, potential toxicity and safety analyses of A. glehni have not been previously investigated. Therefore, this study aimed to evaluate the safety profile of ethanolic extract of A. glehni leaves and stems (EAG) in terms of genotoxicity and subchronic oral animal toxicity under OECD guidelines and GLP conditions. Toxicological assessments were performed at doses of 1,250, 2,500, and 5,000 mg/kg/day in a 13-week oral repeated-dose toxicity study of EAG in male and female SD rats. In addition, an Ames test, an in vitro mammalian chromosomal aberration test, and a micronucleus test were performed. No toxicological changes in clinical signs, body weights, water and food consumption, urinalysis, hematology, clinical biochemistry, gross findings, and histopathological examinations were observed in subchronic oral animal toxicity. In addition, EAG gave negative results when evaluated using in vitro and in vivo genotoxicity tests. In conclusion, the no-observed-adverse-effect level (NOAEL) of EAG was considered to be 5,000 mg/kg/day, and no target organs were identified in both sexes of rats. EAG was also classified as nonmutagenic and nonclastogenic in genotoxicity testing. Collectively, these results show a lack of general toxicity and genotoxicity for EAG that supports clinical work for development as a herbal medicine.
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spelling pubmed-87366942022-01-08 Evaluation of Subchronic Toxicity and Genotoxicity of Ethanolic Extract of Aster glehni Leaves and Stems Lim, Mi Kyung Kim, Ju Yeon Jeong, Jeongho Han, Eun Hye Lee, Sang Ho Lee, Soyeon Kim, Sun-Don Lee, Jinu Evid Based Complement Alternat Med Research Article Aster glehni, a traditional plant on Ulleung Island in the Republic of Korea, has been recognized for its multiple medicinal properties. However, potential toxicity and safety analyses of A. glehni have not been previously investigated. Therefore, this study aimed to evaluate the safety profile of ethanolic extract of A. glehni leaves and stems (EAG) in terms of genotoxicity and subchronic oral animal toxicity under OECD guidelines and GLP conditions. Toxicological assessments were performed at doses of 1,250, 2,500, and 5,000 mg/kg/day in a 13-week oral repeated-dose toxicity study of EAG in male and female SD rats. In addition, an Ames test, an in vitro mammalian chromosomal aberration test, and a micronucleus test were performed. No toxicological changes in clinical signs, body weights, water and food consumption, urinalysis, hematology, clinical biochemistry, gross findings, and histopathological examinations were observed in subchronic oral animal toxicity. In addition, EAG gave negative results when evaluated using in vitro and in vivo genotoxicity tests. In conclusion, the no-observed-adverse-effect level (NOAEL) of EAG was considered to be 5,000 mg/kg/day, and no target organs were identified in both sexes of rats. EAG was also classified as nonmutagenic and nonclastogenic in genotoxicity testing. Collectively, these results show a lack of general toxicity and genotoxicity for EAG that supports clinical work for development as a herbal medicine. Hindawi 2021-12-30 /pmc/articles/PMC8736694/ /pubmed/35003288 http://dx.doi.org/10.1155/2021/1018101 Text en Copyright © 2021 Mi Kyung Lim et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Lim, Mi Kyung
Kim, Ju Yeon
Jeong, Jeongho
Han, Eun Hye
Lee, Sang Ho
Lee, Soyeon
Kim, Sun-Don
Lee, Jinu
Evaluation of Subchronic Toxicity and Genotoxicity of Ethanolic Extract of Aster glehni Leaves and Stems
title Evaluation of Subchronic Toxicity and Genotoxicity of Ethanolic Extract of Aster glehni Leaves and Stems
title_full Evaluation of Subchronic Toxicity and Genotoxicity of Ethanolic Extract of Aster glehni Leaves and Stems
title_fullStr Evaluation of Subchronic Toxicity and Genotoxicity of Ethanolic Extract of Aster glehni Leaves and Stems
title_full_unstemmed Evaluation of Subchronic Toxicity and Genotoxicity of Ethanolic Extract of Aster glehni Leaves and Stems
title_short Evaluation of Subchronic Toxicity and Genotoxicity of Ethanolic Extract of Aster glehni Leaves and Stems
title_sort evaluation of subchronic toxicity and genotoxicity of ethanolic extract of aster glehni leaves and stems
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8736694/
https://www.ncbi.nlm.nih.gov/pubmed/35003288
http://dx.doi.org/10.1155/2021/1018101
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