Human IgE does not bind to human FcRn

The neonatal Fc receptor (FcRn) is known to mediate placental transfer of IgG from mother to unborn. IgE is widely known for triggering immune responses to environmental antigens. Recent evidence suggests FcRn-mediated transplacental passage of IgE during pregnancy. However, direct interaction of Fc...

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Autores principales: Brinkhaus, Maximilian, van der Kooi, Elvera J., Bentlage, Arthur E. H., Ooijevaar-de Heer, Pleuni, Derksen, Ninotska I. L., Rispens, Theo, Vidarsson, Gestur
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8741920/
https://www.ncbi.nlm.nih.gov/pubmed/34996950
http://dx.doi.org/10.1038/s41598-021-03852-1
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author Brinkhaus, Maximilian
van der Kooi, Elvera J.
Bentlage, Arthur E. H.
Ooijevaar-de Heer, Pleuni
Derksen, Ninotska I. L.
Rispens, Theo
Vidarsson, Gestur
author_facet Brinkhaus, Maximilian
van der Kooi, Elvera J.
Bentlage, Arthur E. H.
Ooijevaar-de Heer, Pleuni
Derksen, Ninotska I. L.
Rispens, Theo
Vidarsson, Gestur
author_sort Brinkhaus, Maximilian
collection PubMed
description The neonatal Fc receptor (FcRn) is known to mediate placental transfer of IgG from mother to unborn. IgE is widely known for triggering immune responses to environmental antigens. Recent evidence suggests FcRn-mediated transplacental passage of IgE during pregnancy. However, direct interaction of FcRn and IgE was not investigated. Here, we compared binding of human IgE and IgG variants to recombinant soluble human FcRn with β2-microglobulin (sFcRn) in surface plasmon resonance (SPR) at pH 7.4 and pH 6.0. No interaction was found between human IgE and human sFcRn. These results imply that FcRn can only transport IgE indirectly, and thereby possibly transfer allergenic sensitivity from mother to fetus.
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spelling pubmed-87419202022-01-10 Human IgE does not bind to human FcRn Brinkhaus, Maximilian van der Kooi, Elvera J. Bentlage, Arthur E. H. Ooijevaar-de Heer, Pleuni Derksen, Ninotska I. L. Rispens, Theo Vidarsson, Gestur Sci Rep Article The neonatal Fc receptor (FcRn) is known to mediate placental transfer of IgG from mother to unborn. IgE is widely known for triggering immune responses to environmental antigens. Recent evidence suggests FcRn-mediated transplacental passage of IgE during pregnancy. However, direct interaction of FcRn and IgE was not investigated. Here, we compared binding of human IgE and IgG variants to recombinant soluble human FcRn with β2-microglobulin (sFcRn) in surface plasmon resonance (SPR) at pH 7.4 and pH 6.0. No interaction was found between human IgE and human sFcRn. These results imply that FcRn can only transport IgE indirectly, and thereby possibly transfer allergenic sensitivity from mother to fetus. Nature Publishing Group UK 2022-01-07 /pmc/articles/PMC8741920/ /pubmed/34996950 http://dx.doi.org/10.1038/s41598-021-03852-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Brinkhaus, Maximilian
van der Kooi, Elvera J.
Bentlage, Arthur E. H.
Ooijevaar-de Heer, Pleuni
Derksen, Ninotska I. L.
Rispens, Theo
Vidarsson, Gestur
Human IgE does not bind to human FcRn
title Human IgE does not bind to human FcRn
title_full Human IgE does not bind to human FcRn
title_fullStr Human IgE does not bind to human FcRn
title_full_unstemmed Human IgE does not bind to human FcRn
title_short Human IgE does not bind to human FcRn
title_sort human ige does not bind to human fcrn
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8741920/
https://www.ncbi.nlm.nih.gov/pubmed/34996950
http://dx.doi.org/10.1038/s41598-021-03852-1
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