Robust LC3B lipidation analysis by precisely adjusting autophagic flux

Autophagic flux can be quantified based on the accumulation of lipidated LC3B in the presence of late-stage autophagy inhibitors. This method has been widely applied to identify novel compounds that activate autophagy. Here we scrutinize this approach and show that bafilomycin A1 (BafA) but not chlo...

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Detalles Bibliográficos
Autores principales: Liebl, Martina P., Meister, Sarah C., Frey, Lisa, Hendrich, Kristina, Klemmer, Anja, Wohlfart, Bettina, Untucht, Christopher, Nuber, Judith, Pohl, Christian, Lakics, Viktor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8742033/
https://www.ncbi.nlm.nih.gov/pubmed/34996966
http://dx.doi.org/10.1038/s41598-021-03875-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8742033/
https://www.ncbi.nlm.nih.gov/pubmed/34996966
http://dx.doi.org/10.1038/s41598-021-03875-8

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