Multiplex Gene Tagging with CRISPR-Cas9 for Live-Cell Microscopy and Application to Study the Role of SARS-CoV-2 Proteins in Autophagy, Mitochondrial Dynamics, and Cell Growth

The lack of efficient tools to label multiple endogenous targets in cell lines without staining or fixation has limited our ability to track physiological and pathological changes in cells over time via live-cell studies. Here, we outline the FAST-HDR vector system to be used in combination with CRI...

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Detalles Bibliográficos
Autores principales: Perez-Leal, Oscar, Nixon-Abell, Jonathon, Barrero, Carlos A., Gordon, John C., Oesterling, James, Rico, Mario C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mary Ann Liebert, Inc., publishers 2021
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8742308/
https://www.ncbi.nlm.nih.gov/pubmed/34847745
http://dx.doi.org/10.1089/crispr.2021.0041

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8742308/
https://www.ncbi.nlm.nih.gov/pubmed/34847745
http://dx.doi.org/10.1089/crispr.2021.0041

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