The methylation-independent mismatch repair machinery in Pseudomonas aeruginosa

Over the last 70 years, we’ve all gotten used to an Escherichia coli -centric view of the microbial world. However, genomics, as well as the development of improved tools for genetic manipulation in other species, is showing us that other bugs do things differently, and that we cannot simply extrapo...

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Autores principales: On, Yue Yuan, Welch, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8744996/
https://www.ncbi.nlm.nih.gov/pubmed/34882086
http://dx.doi.org/10.1099/mic.0.001120
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author On, Yue Yuan
Welch, Martin
author_facet On, Yue Yuan
Welch, Martin
author_sort On, Yue Yuan
collection PubMed
description Over the last 70 years, we’ve all gotten used to an Escherichia coli -centric view of the microbial world. However, genomics, as well as the development of improved tools for genetic manipulation in other species, is showing us that other bugs do things differently, and that we cannot simply extrapolate from E. coli to everything else. A particularly good example of this is encountered when considering the mechanism(s) involved in DNA mismatch repair by the opportunistic human pathogen, Pseudomonas aeruginosa (PA). This is a particularly relevant phenotype to examine in PA, since defects in the mismatch repair (MMR) machinery often give rise to the property of hypermutability. This, in turn, is linked with the vertical acquisition of important pathoadaptive traits in the organism, such as antimicrobial resistance. But it turns out that PA lacks some key genes associated with MMR in E. coli , and a closer inspection of what is known (or can be inferred) about the MMR enzymology reveals profound differences compared with other, well-characterized organisms. Here, we review these differences and comment on their biological implications.
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spelling pubmed-87449962022-01-12 The methylation-independent mismatch repair machinery in Pseudomonas aeruginosa On, Yue Yuan Welch, Martin Microbiology (Reading) Reviews Over the last 70 years, we’ve all gotten used to an Escherichia coli -centric view of the microbial world. However, genomics, as well as the development of improved tools for genetic manipulation in other species, is showing us that other bugs do things differently, and that we cannot simply extrapolate from E. coli to everything else. A particularly good example of this is encountered when considering the mechanism(s) involved in DNA mismatch repair by the opportunistic human pathogen, Pseudomonas aeruginosa (PA). This is a particularly relevant phenotype to examine in PA, since defects in the mismatch repair (MMR) machinery often give rise to the property of hypermutability. This, in turn, is linked with the vertical acquisition of important pathoadaptive traits in the organism, such as antimicrobial resistance. But it turns out that PA lacks some key genes associated with MMR in E. coli , and a closer inspection of what is known (or can be inferred) about the MMR enzymology reveals profound differences compared with other, well-characterized organisms. Here, we review these differences and comment on their biological implications. Microbiology Society 2021-12-09 /pmc/articles/PMC8744996/ /pubmed/34882086 http://dx.doi.org/10.1099/mic.0.001120 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
spellingShingle Reviews
On, Yue Yuan
Welch, Martin
The methylation-independent mismatch repair machinery in Pseudomonas aeruginosa
title The methylation-independent mismatch repair machinery in Pseudomonas aeruginosa
title_full The methylation-independent mismatch repair machinery in Pseudomonas aeruginosa
title_fullStr The methylation-independent mismatch repair machinery in Pseudomonas aeruginosa
title_full_unstemmed The methylation-independent mismatch repair machinery in Pseudomonas aeruginosa
title_short The methylation-independent mismatch repair machinery in Pseudomonas aeruginosa
title_sort methylation-independent mismatch repair machinery in pseudomonas aeruginosa
topic Reviews
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8744996/
https://www.ncbi.nlm.nih.gov/pubmed/34882086
http://dx.doi.org/10.1099/mic.0.001120
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