The methylation-independent mismatch repair machinery in Pseudomonas aeruginosa
Over the last 70 years, we’ve all gotten used to an Escherichia coli -centric view of the microbial world. However, genomics, as well as the development of improved tools for genetic manipulation in other species, is showing us that other bugs do things differently, and that we cannot simply extrapo...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Microbiology Society
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8744996/ https://www.ncbi.nlm.nih.gov/pubmed/34882086 http://dx.doi.org/10.1099/mic.0.001120 |
_version_ | 1784630239378276352 |
---|---|
author | On, Yue Yuan Welch, Martin |
author_facet | On, Yue Yuan Welch, Martin |
author_sort | On, Yue Yuan |
collection | PubMed |
description | Over the last 70 years, we’ve all gotten used to an Escherichia coli -centric view of the microbial world. However, genomics, as well as the development of improved tools for genetic manipulation in other species, is showing us that other bugs do things differently, and that we cannot simply extrapolate from E. coli to everything else. A particularly good example of this is encountered when considering the mechanism(s) involved in DNA mismatch repair by the opportunistic human pathogen, Pseudomonas aeruginosa (PA). This is a particularly relevant phenotype to examine in PA, since defects in the mismatch repair (MMR) machinery often give rise to the property of hypermutability. This, in turn, is linked with the vertical acquisition of important pathoadaptive traits in the organism, such as antimicrobial resistance. But it turns out that PA lacks some key genes associated with MMR in E. coli , and a closer inspection of what is known (or can be inferred) about the MMR enzymology reveals profound differences compared with other, well-characterized organisms. Here, we review these differences and comment on their biological implications. |
format | Online Article Text |
id | pubmed-8744996 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Microbiology Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-87449962022-01-12 The methylation-independent mismatch repair machinery in Pseudomonas aeruginosa On, Yue Yuan Welch, Martin Microbiology (Reading) Reviews Over the last 70 years, we’ve all gotten used to an Escherichia coli -centric view of the microbial world. However, genomics, as well as the development of improved tools for genetic manipulation in other species, is showing us that other bugs do things differently, and that we cannot simply extrapolate from E. coli to everything else. A particularly good example of this is encountered when considering the mechanism(s) involved in DNA mismatch repair by the opportunistic human pathogen, Pseudomonas aeruginosa (PA). This is a particularly relevant phenotype to examine in PA, since defects in the mismatch repair (MMR) machinery often give rise to the property of hypermutability. This, in turn, is linked with the vertical acquisition of important pathoadaptive traits in the organism, such as antimicrobial resistance. But it turns out that PA lacks some key genes associated with MMR in E. coli , and a closer inspection of what is known (or can be inferred) about the MMR enzymology reveals profound differences compared with other, well-characterized organisms. Here, we review these differences and comment on their biological implications. Microbiology Society 2021-12-09 /pmc/articles/PMC8744996/ /pubmed/34882086 http://dx.doi.org/10.1099/mic.0.001120 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution. |
spellingShingle | Reviews On, Yue Yuan Welch, Martin The methylation-independent mismatch repair machinery in Pseudomonas aeruginosa |
title | The methylation-independent mismatch repair machinery in Pseudomonas aeruginosa
|
title_full | The methylation-independent mismatch repair machinery in Pseudomonas aeruginosa
|
title_fullStr | The methylation-independent mismatch repair machinery in Pseudomonas aeruginosa
|
title_full_unstemmed | The methylation-independent mismatch repair machinery in Pseudomonas aeruginosa
|
title_short | The methylation-independent mismatch repair machinery in Pseudomonas aeruginosa
|
title_sort | methylation-independent mismatch repair machinery in pseudomonas aeruginosa |
topic | Reviews |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8744996/ https://www.ncbi.nlm.nih.gov/pubmed/34882086 http://dx.doi.org/10.1099/mic.0.001120 |
work_keys_str_mv | AT onyueyuan themethylationindependentmismatchrepairmachineryinpseudomonasaeruginosa AT welchmartin themethylationindependentmismatchrepairmachineryinpseudomonasaeruginosa AT onyueyuan methylationindependentmismatchrepairmachineryinpseudomonasaeruginosa AT welchmartin methylationindependentmismatchrepairmachineryinpseudomonasaeruginosa |