Cargando…

Rapid genome editing by CRISPR-Cas9-POLD3 fusion

Precision CRISPR gene editing relies on the cellular homology-directed DNA repair (HDR) to introduce custom DNA sequences to target sites. The HDR editing efficiency varies between cell types and genomic sites, and the sources of this variation are incompletely understood. Here, we have studied the...

Descripción completa

Detalles Bibliográficos
Autores principales:	Reint, Ganna, Li, Zhuokun, Labun, Kornel, Keskitalo, Salla, Soppa, Inkeri, Mamia, Katariina, Tolo, Eero, Szymanska, Monika, Meza-Zepeda, Leonardo A, Lorenz, Susanne, Cieslar-Pobuda, Artur, Hu, Xian, Bordin, Diana L, Staerk, Judith, Valen, Eivind, Schmierer, Bernhard, Varjosalo, Markku, Taipale, Jussi, Haapaniemi, Emma
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	eLife Sciences Publications, Ltd 2021
Materias:	Cell Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8747517/ https://www.ncbi.nlm.nih.gov/pubmed/34898428 http://dx.doi.org/10.7554/eLife.75415

Ejemplares similares

Protocol for editing fibroblasts with in vitro transcribed Cas9 mRNA and profile off-target editing by optimized GUIDE-seq
por: Li, Zhuokun, et al.
Publicado: (2023)

Deep conservation of ribosome stall sites across RNA processing genes
por: Chyżyńska, Katarzyna, et al.
Publicado: (2021)

CHOPCHOP v2: a web tool for the next generation of CRISPR genome engineering
por: Labun, Kornel, et al.
Publicado: (2016)

Accurate analysis of genuine CRISPR editing events with ampliCan
por: Labun, Kornel, et al.
Publicado: (2019)

ORFik: a comprehensive R toolkit for the analysis of translation
por: Tjeldnes, Håkon, et al.
Publicado: (2021)

Long-read single-molecule RNA structure sequencing using nanopore
por: Bizuayehu, Teshome Tilahun, et al.
Publicado: (2022)

CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing
por: Labun, Kornel, et al.
Publicado: (2019)

tailfindr: alignment-free poly(A) length measurement for Oxford Nanopore RNA and DNA sequencing
por: Krause, Maximilian, et al.
Publicado: (2019)

Reply to “CRISPR screens are feasible in TP53 wild‐type cells”
por: Haapaniemi, Emma, et al.
Publicado: (2019)

Roles of human POLD1 and POLD3 in genome stability
por: Tumini, Emanuela, et al.
Publicado: (2016)

Role of ion channels in regulating Ca(2+) homeostasis during the interplay between immune and cancer cells
por: Bose, T, et al.
Publicado: (2015)

Novel TMEM173 Mutation and the Role of Disease Modifying Alleles
por: Keskitalo, Salla, et al.
Publicado: (2019)

Editorial: Transdetermination, transdifferentiation, and reprogramming of cells: In vitro and in vivo strategies
por: Cieslar-Pobuda, Artur, et al.
Publicado: (2023)

Physical and functional interactome atlas of human receptor tyrosine kinases
por: Salokas, Kari, et al.
Publicado: (2022)

Role of POLE and POLD1 in familial cancer
por: Mur, Pilar, et al.
Publicado: (2020)

POLE/POLD1 mutation and tumor immunotherapy
por: Ma, Xiaoting, et al.
Publicado: (2022)

Characterization of Expanded Gamma Delta T Cells from Atypical X-SCID Patient Reveals Preserved Function and IL2RG-Mediated Signaling
por: Tuovinen, Elina A., et al.
Publicado: (2022)

CTCF Mediates Replicative Senescence Through POLD1
por: Hou, Yuli, et al.
Publicado: (2021)

Prospects of POLD1 in Human Cancers: A Review
por: Gola, Michał, et al.
Publicado: (2023)

The Impact of ETV6-NTRK3 Oncogenic Gene Fusions on Molecular and Signaling Pathway Alterations
por: Kinnunen, Matias, et al.
Publicado: (2023)

Dominant TOM1 mutation associated with combined immunodeficiency and autoimmune disease
por: Keskitalo, Salla, et al.
Publicado: (2019)

A Comprehensive Prognostic Analysis of POLD1 in Hepatocellular Carcinoma
por: Tang, Hui, et al.
Publicado: (2022)

IRF2BP2 Mutation Is Associated with Increased STAT1 and STAT5 Activation in Two Family Members with Inflammatory Conditions and Lymphopenia
por: Palmroth, Maaria, et al.
Publicado: (2021)

Elevated expression of POLD1 is associated with poor prognosis in breast cancer
por: Qin, Qinghong, et al.
Publicado: (2018)

Scrutinizing Deleterious Nonsynonymous SNPs and Their Effect on Human POLD1 Gene
por: Bappy, Md. Nazmul Islam, et al.
Publicado: (2022)

Recommendations for the classification of germline variants in the exonuclease domain of POLE and POLD1
por: Mur, Pilar, et al.
Publicado: (2023)

NADPH Oxidases: Insights into Selected Functions and Mechanisms of Action in Cancer and Stem Cells
por: Skonieczna, Magdalena, et al.
Publicado: (2017)

ROS and Oxidative Stress in Stem Cells
por: Cieślar-Pobuda, Artur, et al.
Publicado: (2017)

Targeted Metabolic Profiling of Methionine Cycle Metabolites and Redox Thiol Pools in Mammalian Plasma, Cells and Urine
por: Behringer, Sidney, et al.
Publicado: (2019)

Human POLD1 modulates cell cycle progression and DNA damage repair
por: Song, Jing, et al.
Publicado: (2015)

Risk of colorectal cancer for carriers of a germline mutation in POLE or POLD1
por: Buchanan, Daniel D., et al.
Publicado: (2017)

Detection of Allelic Variants of The POLE and POLD1 Genes in Colorectal Cancer Patients
por: Pätzold, LA, et al.
Publicado: (2017)

E2F1 mediates the downregulation of POLD1 in replicative senescence
por: Gao, Shichao, et al.
Publicado: (2019)

POLE and POLD1 pathogenic variants in the proofreading domain in papillary thyroid cancer
por: Siraj, Abdul K, et al.
Publicado: (2020)

Assessment of POLE and POLD1 mutations as prognosis and immunotherapy biomarkers for stomach adenocarcinoma
por: Zhu, Mingyu, et al.
Publicado: (2022)

The expression pattern of PFKFB3 enzyme distinguishes between induced-pluripotent stem cells and cancer stem cells
por: Cieślar-Pobuda, Artur, et al.
Publicado: (2015)

Inhibition of miR301 enhances Akt-mediated cell proliferation by accumulation of PTEN in nucleus and its effects on cell-cycle regulatory proteins
por: Jain, Mayur V., et al.
Publicado: (2016)

POLD1: Central mediator of DNA replication and repair, and implication in cancer and other pathologies
por: Nicolas, Emmanuelle, et al.
Publicado: (2016)

Pold3 is required for genomic stability and telomere integrity in embryonic stem cells and meiosis
por: Zhou, Zhongcheng, et al.
Publicado: (2018)

DNA polymerase POLD1 promotes proliferation and metastasis of bladder cancer by stabilizing MYC
por: Wang, Yejinpeng, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_bgva8q4o7qg1hil64dpmcg2r5f