Translation initiation from sequence variants of the bacteriophage T7 g10RBS in Escherichia coli and Agrobacterium fabrum

BACKGROUND: The bacteriophage T7 gene 10 ribosome binding site (g10RBS) has long been used for robust expression of recombinant proteins in Escherichia coli. This RBS consists of a Shine–Dalgarno (SD) sequence augmented by an upstream translational “enhancer” (Enh) element, supporting protein produc...

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Detalles Bibliográficos
Autores principales: Benedict, Alex B., Chamberlain, Joshua D., Calvopina, Diana G., Griffitts, Joel S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2021
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8748333/
https://www.ncbi.nlm.nih.gov/pubmed/34743270
http://dx.doi.org/10.1007/s11033-021-06891-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8748333/
https://www.ncbi.nlm.nih.gov/pubmed/34743270
http://dx.doi.org/10.1007/s11033-021-06891-z

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