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In vitro antioxidant extracts evaluation from the residue of the Hevea brasiliensis seed

The antioxidants used in the food industry are essential to inhibit the formation of free radicals, preserving the existing properties in the different matrices. However, the insecurity of the synthetic antioxidants regarding human health propels search for natural substrates with potential antioxid...

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Autores principales: Oleinik, Giovanna, Dario, Priscila Paola, de Morais Gasperin, Katiane, Benvegnú, Dalila Moter, Lima, Fernanda Oliveira, Soares, Letiére Cabreira, Gallina, André Lazarin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8748519/
https://www.ncbi.nlm.nih.gov/pubmed/35013381
http://dx.doi.org/10.1038/s41598-021-04017-w
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author Oleinik, Giovanna
Dario, Priscila Paola
de Morais Gasperin, Katiane
Benvegnú, Dalila Moter
Lima, Fernanda Oliveira
Soares, Letiére Cabreira
Gallina, André Lazarin
author_facet Oleinik, Giovanna
Dario, Priscila Paola
de Morais Gasperin, Katiane
Benvegnú, Dalila Moter
Lima, Fernanda Oliveira
Soares, Letiére Cabreira
Gallina, André Lazarin
author_sort Oleinik, Giovanna
collection PubMed
description The antioxidants used in the food industry are essential to inhibit the formation of free radicals, preserving the existing properties in the different matrices. However, the insecurity of the synthetic antioxidants regarding human health propels search for natural substrates with potential antioxidant activity as an alternative to synthetic compounds. In this way, the work had as objective obtaining extracts from the seed pomace of the Hevea brasiliensis (rubber tree), relating the contents of flavonoids and total phenols in the application as an antioxidant. The methodology consisted of the extraction using four solvents, varying extractive methods, time, and seed concentrations. The antioxidant activity in vitro was evaluated by capturing the DPPH (2,2-diphenyl-1-picryl-hydrazil) radical. The optimized results demonstrate that the aqueous extracts produced in the Soxhlet in the concentrations of 85 g L(−1) and retention time of 4 h reached 37.73 ± 1.69% in the antioxidant tests of the free radical DPPH capture, 1405.15 mg EAC 100 g(−1) in the quantification of phenolic compounds and 223.34 mg 100 g(−1) of total flavonoids. Thus, this work may contribute to the realization of studies and future research for characterization and identification concerning which phenolic compounds and flavonoids attribute the antioxidant characteristic to the extracts produced, enabling the discovery of products with high added value in the production chain. In addition, because the water used as a solvent showed greater antioxidant potential between the extracts, the non-toxic and environmentally friendly character is highlighted, allowing a wide variety of applications in the food industry.
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spelling pubmed-87485192022-01-11 In vitro antioxidant extracts evaluation from the residue of the Hevea brasiliensis seed Oleinik, Giovanna Dario, Priscila Paola de Morais Gasperin, Katiane Benvegnú, Dalila Moter Lima, Fernanda Oliveira Soares, Letiére Cabreira Gallina, André Lazarin Sci Rep Article The antioxidants used in the food industry are essential to inhibit the formation of free radicals, preserving the existing properties in the different matrices. However, the insecurity of the synthetic antioxidants regarding human health propels search for natural substrates with potential antioxidant activity as an alternative to synthetic compounds. In this way, the work had as objective obtaining extracts from the seed pomace of the Hevea brasiliensis (rubber tree), relating the contents of flavonoids and total phenols in the application as an antioxidant. The methodology consisted of the extraction using four solvents, varying extractive methods, time, and seed concentrations. The antioxidant activity in vitro was evaluated by capturing the DPPH (2,2-diphenyl-1-picryl-hydrazil) radical. The optimized results demonstrate that the aqueous extracts produced in the Soxhlet in the concentrations of 85 g L(−1) and retention time of 4 h reached 37.73 ± 1.69% in the antioxidant tests of the free radical DPPH capture, 1405.15 mg EAC 100 g(−1) in the quantification of phenolic compounds and 223.34 mg 100 g(−1) of total flavonoids. Thus, this work may contribute to the realization of studies and future research for characterization and identification concerning which phenolic compounds and flavonoids attribute the antioxidant characteristic to the extracts produced, enabling the discovery of products with high added value in the production chain. In addition, because the water used as a solvent showed greater antioxidant potential between the extracts, the non-toxic and environmentally friendly character is highlighted, allowing a wide variety of applications in the food industry. Nature Publishing Group UK 2022-01-10 /pmc/articles/PMC8748519/ /pubmed/35013381 http://dx.doi.org/10.1038/s41598-021-04017-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Oleinik, Giovanna
Dario, Priscila Paola
de Morais Gasperin, Katiane
Benvegnú, Dalila Moter
Lima, Fernanda Oliveira
Soares, Letiére Cabreira
Gallina, André Lazarin
In vitro antioxidant extracts evaluation from the residue of the Hevea brasiliensis seed
title In vitro antioxidant extracts evaluation from the residue of the Hevea brasiliensis seed
title_full In vitro antioxidant extracts evaluation from the residue of the Hevea brasiliensis seed
title_fullStr In vitro antioxidant extracts evaluation from the residue of the Hevea brasiliensis seed
title_full_unstemmed In vitro antioxidant extracts evaluation from the residue of the Hevea brasiliensis seed
title_short In vitro antioxidant extracts evaluation from the residue of the Hevea brasiliensis seed
title_sort in vitro antioxidant extracts evaluation from the residue of the hevea brasiliensis seed
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8748519/
https://www.ncbi.nlm.nih.gov/pubmed/35013381
http://dx.doi.org/10.1038/s41598-021-04017-w
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