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High-throughput formation and image-based analysis of basal-in mammary organoids in 384-well plates

This manuscript describes a new method for forming basal-in MCF10A organoids using commercial 384-well ultra-low attachment (ULA) microplates and the development of associated live-cell imaging and automated analysis protocols. The use of a commercial 384-well ULA platform makes this method more bro...

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Detalles Bibliográficos
Autores principales: Lee, Soojung, Chang, Jonathan, Kang, Sung-Min, Parigoris, Eric, Lee, Ji-Hoon, Huh, Yun Suk, Takayama, Shuichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8748891/
https://www.ncbi.nlm.nih.gov/pubmed/35013350
http://dx.doi.org/10.1038/s41598-021-03739-1