High-throughput in vitro processing of human primary microRNA by the recombinant microprocessor

We describe a protocol to conduct a high-throughput in vitro processing assay, using 1,881 human primary microRNAs (pri-miRNAs) and recombinant Microprocessor complex, followed by deep sequencing library generation. This comprehensive approach allows the mapping of cleavage sites and the measurement...

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Detalles Bibliográficos
Autores principales: Kim, Kijun, Kim, V. Narry
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8749294/
https://www.ncbi.nlm.nih.gov/pubmed/35036952
http://dx.doi.org/10.1016/j.xpro.2021.101042

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8749294/
https://www.ncbi.nlm.nih.gov/pubmed/35036952
http://dx.doi.org/10.1016/j.xpro.2021.101042

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